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Western Blot (WB) (Western blot analysis of extracts from HeLa cells, COLO cells, MCF-7 cells and HepG2 cells, using RED antibody.)

RED Antibody | anti-RED antibody

RED Antibody

Gene Names
IK; RED; RER; CSA2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
RED; RED Antibody; chondrosarcoma-associated protein 2; CSA2; cytokine IK; IK cytokine; down-regulator of HLA II; IK factor; prer protein; RD element; Red; RER; anti-RED antibody
Ordering
For Research Use Only!
Reactivity
Human, Mouse, Rat
Specificity
RED antibody detects endogenous levels of total RED protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
557
Applicable Applications for anti-RED antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
ELISA: 1:20000
Immunogen
The antiserum was produced against synthesized peptide derived from internal of human RED.
Preparation and Storage
Store at-20 degree C for 1 year.

Western Blot (WB)

(Western blot analysis of extracts from HeLa cells, COLO cells, MCF-7 cells and HepG2 cells, using RED antibody.)

Western Blot (WB) (Western blot analysis of extracts from HeLa cells, COLO cells, MCF-7 cells and HepG2 cells, using RED antibody.)
References
Assier E., Gene 230:145-154(1999). Krief P., Oncogene 9:3449-3456(1994). Matsuoka S., Science 316:1160-1166(2007).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
65,602 Da
NCBI Official Full Name
protein Red
NCBI Official Synonym Full Names
IK cytokine
NCBI Official Symbol
IK
NCBI Official Synonym Symbols
RED; RER; CSA2
NCBI Protein Information
protein Red
UniProt Protein Name
Protein Red
Protein Family
UniProt Gene Name
IK

NCBI Description

The protein encoded by this gene was identified by its RED repeat, a stretch of repeated arginine, glutamic acid and aspartic acid residues. The protein localizes to discrete dots within the nucleus, excluding the nucleolus. Its function is unknown. This gene maps to chromosome 5; however, a pseudogene may exist on chromosome 2. [provided by RefSeq, Jul 2008]

Uniprot Description

Involved in pre-mRNA splicing as a component of the spliceosome (PubMed:28781166). Auxiliary spliceosomal protein that regulates selection of alternative splice sites in a small set of target pre-mRNA species (Probable). Required for normal mitotic cell cycle progression (PubMed:22351768, PubMed:24252166). Recruits MAD1L1 and MAD2L1 to kinetochores, and is required to trigger the spindle assembly checkpoint (PubMed:22351768). Required for normal accumulation of SMU1 (PubMed:24945353).

Research Articles on RED

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Product Notes

The RED ik (Catalog #AAA855515) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The RED Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RED can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500~1:1000 ELISA: 1:20000. Researchers should empirically determine the suitability of the RED ik for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RED, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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