Mouse anti-Human ADRM1 Monoclonal Antibody | anti-ADRM1 antibody

ADRM1 (GP110, Proteasomal Ubiquitin Receptor ADRM1, 110kD Cell Membrane Glycoprotein, Adhesion-regulating Molecule 1, Proteasome Regulatory Particle Non-ATPase 13, Rpn13 Homolog, MGC29536) (Biotin)

Cat. Num. AAA6140508

• Gene Names: ADRM1; ARM1; ARM-1; GP110
• Reactivity: Human
• Applications: ELISA, Immunofluorescence, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ADRM1; Monoclonal Antibody; ADRM1 (GP110; Proteasomal Ubiquitin Receptor ADRM1; 110kD Cell Membrane Glycoprotein; Adhesion-regulating Molecule 1; Proteasome Regulatory Particle Non-ATPase 13; Rpn13 Homolog; MGC29536) (Biotin); anti-ADRM1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b,k
• Clone Number: 3C6
• Specificity: Recognizes human ADRM1.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-ADRM1 antibody: ELISA (EIA), Immunofluorescence (IF), Western Blot (WB)
• Application Notes: IF: 10ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa18-408 from human ADRM1 (AAH17245) with GST tag. MW of the GST tag alone is 26kD.
• Conjugate: Biotin
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (69.01kD).)

Western Blot (WB)

(ADRM1 monoclonal antibody Western Blot analysis of ADRM1 expression in HeLa.)

Western Blot (WB)

(Western Blot analysis of ADRM1 expression in transfected 293T cell line by ADRM1 monoclonal antibody Lane 1: ADRM1 transfected lysate (42.2kD). Lane 2: Non-transfected lysate.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to ADRM1 on HeLa cell. [antibody concentration 10ug/ml].)

Immunoprecipitation (IP)

(Immunoprecipitation of ADRM1 transfected lysate using ADRM1 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with ADRM1 monoclonal antibody.)

Testing Data

(Detection limit for recombinant GST tagged ADRM1 is ~0.1ng/ml as a capture antibody.)

Product Categories/Family for anti-ADRM1 antibody

Antibodies; Ubiquitin

NCBI and Uniprot Product Information

• NCBI GI #: 33878567
• NCBI GeneID: 11047
• Molecular Weight: 42,153 Da
• NCBI Official Full Name: Homo sapiens adhesion regulating molecule 1, mRNA
• NCBI Official Synonym Full Names: adhesion regulating molecule 1
• NCBI Official Symbol: ADRM1
• NCBI Official Synonym Symbols: ARM1; ARM-1; GP110
• NCBI Protein Information: proteasomal ubiquitin receptor ADRM1

NCBI Description

This gene encodes a member of the adhesion regulating molecule 1 protein family. The encoded protein is a component of the proteasome where it acts as a ubiquitin receptor and recruits the deubiquitinating enzyme, ubiquitin carboxyl-terminal hydrolase L5. Increased levels of the encoded protein are associated with increased cell adhesion, which is likely an indirect effect of this intracellular protein. Dysregulation of this gene has been implicated in carcinogenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]