Mouse Adrenergic Receptor Kinase, beta 1 Monoclonal Antibody | anti-ADRBK1 antibody

Adrenergic Receptor Kinase, beta 1 (bARK1, B1 AR, ARK1 beta, G Protein Coupled Receptor Kinase 2, GPCR K2, GRK2)

Cat. Num. AAA606296

• Gene Names: ADRBK1; GRK2; BARK1; FLJ16718; BETA-ARK1
• Reactivity: Human, Mammal, Rat
• Applications: ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Adrenergic Receptor Kinase; beta 1; Monoclonal Antibody; beta 1 (bARK1; B1 AR; ARK1 beta; G Protein Coupled Receptor Kinase 2; GPCR K2; GRK2); Anti -Adrenergic Receptor Kinase; anti-ADRBK1 antibody

• Host: Mouse
• Reactivity: Human, Mammal, Rat
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 3G3
• Specificity: Reacts with mammalian b-adrenergic receptor kinase.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide, before the addition of glycerol to 40%.

• Applicable Applications for anti-ADRBK1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry.; Dilution: Immunofluorescence: 3-10ug/ml; ELISA: 0.1-1ug/ml; Western Blot: 1-5ug/ml; Immunohistochemistry: 10ug/ml; Immunoprecipitation: 10ug/IP reaction
• Immunogen: Recombinant b-adrenergic receptor kinase.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-ADRBK1 antibody

G protein-coupled receptor kinases, a more recently discovered family, have the unique features of phosphorylating G-protein coupled receptors only when they are in their active or stimulated conformations. The range of signals acting through G protein-coupled receptors includes numerous neurotransmitters, chemoattractants, hormones, cytokines, and sensory stimuli such as photons or odorants. Structurally, the G-protein coupled receptor kinases show significant amino acid similarity to other kinases only in the centrally located catalytic domain of about 240 amino acid residues. Rapid phosphorylation of many G protein coupled receptors accompanies stimulus-driven desensitization. There are two types of kinases known to mediate these modifications: second messenger kinases and novel G protein-coupled receptor kinases. G protein-coupled receptor kinase family members include rhodopsin kinase, two isoforms of beta-adrenergic receptor kinase (betaARK), a gene cloned from the Huntington's disease locus on chromosome 4, GRK5, and several homologs from Drosophila. BetaARK1 is widely distributed throughout the mammalian central nervous system, especially at synapses, suggesting broader roles in regulating a wide variety of neurotransmitter receptors. BetaARK1 was identified as a kinase that phosphorylates betaARs in an agonist-dependent manner. Beta ARK1 has properties similar to the rhodopsin kinase in that BetaARK is also insensitive to cAMP, cGMP, and Ca2+ and does not phosphorylate histone. BetaARK is capable of phosphorylating photoexcited rhodopsin, although the betaAR is a better substrate for betaARK than rhodopsin. Both rhodopsin kinase and betaARK are inhibited by salts such as 0.1M NaCl, low concentrations of Zn (0.1-1mM), detergents such as digitonin, and polyanions such as dextransulfate and polyaspartic acid. Both kinases use ATP as the preferred substrate over GTP. In the case of betaARK, the kinase interacts with G protein beta-gamma subunits as well as with receptors, and the ternary complex of agonist bound receptor, kinase, and beta-gamma subunits is considered to be an active form for the phosphorylation of receptors. Multiple independent lines of evidence indicate that the phosphorylation of receptors by G-protein coupled receptor kinases in vivo regulates their function.

Product Categories/Family for anti-ADRBK1 antibody

Antibodies; Abs to G Protein Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 23272739
• NCBI GeneID: 156
• UniProt Accession #: P25098; Q13837; Q6GTT3; B0ZBE1; Q59F73
• Molecular Weight: 79,574 Da
• NCBI Official Full Name: Adrenergic, beta, receptor kinase 1
• NCBI Official Synonym Full Names: adrenergic, beta, receptor kinase 1
• NCBI Official Symbol: ADRBK1
• NCBI Official Synonym Symbols: GRK2; BARK1; FLJ16718; BETA-ARK1
• NCBI Protein Information: beta-adrenergic receptor kinase 1; beta-ARK-1; OTTHUMP00000235171; G-protein coupled receptor kinase 2
• UniProt Protein Name: Beta-adrenergic receptor kinase 1
• UniProt Gene Name: ADRBK1
• UniProt Synonym Gene Names: BARK; BARK1; GRK2
• UniProt Entry Name: ARBK1_HUMAN

NCBI Description

The product of this gene phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. This protein is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart. [provided by RefSeq]

Uniprot Description

GRK2: a ubiquitous protein kinase of the GRK family. Phosphorylates the beta-2-adrenergic receptor and related G-protein-coupled receptors. Appears to mediate agonist-specific desensitization observed at high agonist concentrations. Expression level is consistently elevated in chronic human heart failure. Mouse models of severe heart failure have been used to demonstrate that inhibition of BARK1 with a peptide inhibitor is sufficient to increase mean survival, reduce dialation and improve cardiac function.

Protein type: Kinase, protein; EC 2.7.11.15; Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; AGC group; GRK family; BARK subfamily

Chromosomal Location of Human Ortholog: 11q13.1

Cellular Component: membrane; cytoplasm; plasma membrane; cytosol

Molecular Function: G-protein coupled receptor kinase activity; protein binding; beta-adrenergic receptor kinase activity; Edg-2 lysophosphatidic acid receptor binding; alpha-2A adrenergic receptor binding; ATP binding; protein kinase activity

Biological Process: epidermal growth factor receptor signaling pathway; desensitization of G-protein coupled receptor protein signaling pathway; tachykinin signaling pathway; fibroblast growth factor receptor signaling pathway; acetylcholine receptor signaling, muscarinic pathway; nerve growth factor receptor signaling pathway; heart development; peptidyl-threonine phosphorylation; signal transduction; negative regulation of striated muscle contraction; peptidyl-serine phosphorylation; negative regulation of the force of heart contraction by chemical signal; phospholipase C activation; receptor internalization; innate immune response; positive regulation of catecholamine secretion; cardiac muscle contraction