Western Blot (WB)
(ADH5 monoclonal antibody (M01), clone 12F7. Western Blot analysis of ADH5 expression in K-562.)
Mouse ADH5 Monoclonal Antibody | anti-ADH5 antibody
ADH5 (Alcohol Dehydrogenase 5 (Class III), chi Polypeptide, ADH-3, ADHX, FDH, GSNOR) (HRP)
Gene Names
ADH5; FDH; ADHX; ADH-3; FALDH; GSNOR; GSH-FDH; HEL-S-60p
Applications
Western Blot
Purity
Purified
Synonyms
ADH5; Monoclonal Antibody; ADH5 (Alcohol Dehydrogenase 5 (Class III); chi Polypeptide; ADH-3; ADHX; FDH; GSNOR) (HRP); Alcohol Dehydrogenase 5 (Class III); GSNOR; anti-ADH5 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
12F7
Specificity
Recognizes ADH5.
Purity/Purification
Purified
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Sequence Length
374
Applicable Applications for anti-ADH5 antibody
Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
ADH5 (AAH14665, 1aa-374aa) full-length recombinant protein with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MANEVIKCKAAVAWEAGKPLSIEEIEVAPPKAHEVRIKIIATAVCHTDAYTLSGADPEGCFPVILGHEGAGIVESVGEGVTKLKAGDTVIPLYIPQCGECKFCLNPKTNLCQKIRVTQGKGLMPDGTSRFTCKGKTILHYMGTSTFSEYTVVADISVAKIDPLAPLDKVCLLGCGISTGYGAAVNTAKLEPGSVCAVFGLGGVGLAVIMGCKVAGASRIIGVDINKDKFARAKEFGATECINPQDFSKPIQEVLIEMTDGGVDYSFECIGNVKVMRAALEACHKGWGVSVVVGVAASGGEIATRPFQLVTGRTWKGTAFGGWKSVESVPKLVSEYMSKKIKVDEFVTHNLSFDEINKAFELMHSGKSIRTVVKI
Conjugate
HRP
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(ADH5 monoclonal antibody (M01), clone 12F7. Western Blot analysis of ADH5 expression in K-562.)
Western Blot (WB)
(ADH5 monoclonal antibody (M01), clone 12F7. Western Blot analysis of ADH5 expression in K-562.)
Testing Data
(Detection limit for recombinant GST tagged ADH5 is 1 ng/ml as a capture antibody.)
Testing Data
(Detection limit for recombinant GST tagged ADH5 is 1 ng/ml as a capture antibody.)
Product Categories/Family for anti-ADH5 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Alcohol dehydrogenase 5 (class III), chi polypeptide
NCBI Official Synonym Full Names
alcohol dehydrogenase 5 (class III), chi polypeptide
NCBI Official Symbol
ADH5
NCBI Official Synonym Symbols
FDH; ADHX; ADH-3; FALDH; GSNOR; GSH-FDH; HEL-S-60p
NCBI Protein Information
alcohol dehydrogenase class-3
Protein Family
NCBI Description
This gene encodes a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. The encoded protein forms a homodimer. It has virtually no activity for ethanol oxidation, but exhibits high activity for oxidation of long-chain primary alcohols and for oxidation of S-hydroxymethyl-glutathione, a spontaneous adduct between formaldehyde and glutathione. This enzyme is an important component of cellular metabolism for the elimination of formaldehyde, a potent irritant and sensitizing agent that causes lacrymation, rhinitis, pharyngitis, and contact dermatitis. The human genome contains several non-transcribed pseudogenes related to this gene. [provided by RefSeq, Oct 2008]
Research Articles on ADH5
Similar Products
Product Notes
The ADH5 (Catalog #AAA6183024) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's ADH5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the ADH5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ADH5, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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