Mouse anti-Human Adenosine Deaminase, RNA-specific, B1 Monoclonal Antibody | anti-ADARB1 antibody

Adenosine Deaminase, RNA-specific, B1 (ADARB1, ADAR2, Double-stranded RNA-specific Editase 1, DRABA2, DRADA2, dsRNA Adenosine Deaminase, RNA Editase, RNA-editing Deaminase 1, RED1, RNA-editing Enzyme 1)

Cat. Num. AAA607515

• Gene Names: ADARB1; RED1; ADAR2; DRABA2; DRADA2
• Reactivity: Human
• Applications: Western Blot, Gel Super Shift Assay
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Adenosine Deaminase; RNA-specific; B1; Monoclonal Antibody; B1 (ADARB1; ADAR2; Double-stranded RNA-specific Editase 1; DRABA2; DRADA2; dsRNA Adenosine Deaminase; RNA Editase; RNA-editing Deaminase 1; RED1; RNA-editing Enzyme 1); Anti -Adenosine Deaminase; anti-ADARB1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 9j17
• Specificity: Recognizes human ADARB1.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.

• Applicable Applications for anti-ADARB1 antibody: Western Blot (WB), Gel Shift Assay (GS/EMSA)
• Application Notes: Suitable for use in Western Blot and Dot Blot.
• Immunogen: Partial recombinant sequence from the N-terminus of human ADARB1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

Related Product Information for anti-ADARB1 antibody

The mammalian Adenosine Deaminases Acting on RNA (ADAR) constitute a family of sequence-related proteins involved in pre-mRNA editing of nuclear transcripts through site-speci c adenosine modi cation. ADARs are not active on adenosine-mononucleotide and are only distantly related by sequence to adenosine and AMP deaminases involved in purine metabolism. The ADARs convert certain adenosine bases in an RNA transcript into inosines by removing an amino group involved in Watson-Crick base pairing. The ADARs recognize a double stranded RNA (dsRNA) structure in the transcript that is formed between the editing site complementary sequence (ECS), usually located in the downstream 3' intron of the transcript, and the sequence to be edited. The ADAR family of proteins consists of ADAR1 and ADAR2 which are expressed in many tissues and ADAR3 which is expressed in the brain. Regulated levels of ADAR1 expression are critical for embryonic erythropoiesis in the liver. Results show a requirement of regulated increase in the ADAR1 expression in liver at E12 and E13 stages. Failure to increase ADAR1 expression may result in underediting of the RNA of currently unknown target genes, which in turn affects proliferation and/or differentiation of erythrocytes. The absence of mature erythrocytes would lead to tissue hypoxia and the eventual death of the embryo. The best characterized target transcripts that undergo RNA editing by ADARs encode glutamate-sensitive ion channels that are expressed by neurons of the central nervous system.

Product Categories/Family for anti-ADARB1 antibody

Antibodies; Abs to Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 119629766
• NCBI GeneID: 104
• UniProt Accession #: O43263; P78563; Q4AE77; Q4AE81; Q6P0M9; Q86XN4; Q8NFA1; Q8TCX7
• Molecular Weight: 75,970 Da
• NCBI Official Full Name: adenosine deaminase, RNA-specific, B1 (RED1 homolog rat), isoform CRA_e
• NCBI Official Synonym Full Names: adenosine deaminase, RNA-specific, B1
• NCBI Official Symbol: ADARB1
• NCBI Official Synonym Symbols: RED1; ADAR2; DRABA2; DRADA2
• NCBI Protein Information: double-stranded RNA-specific editase 1; RNA editase; RED1 homolog; OTTHUMP00000115339; OTTHUMP00000115340; OTTHUMP00000115341; OTTHUMP00000115342; OTTHUMP00000174981; OTTHUMP00000214128; RNA-editing enzyme 1; RNA editing deaminase 1; RNA-editing deaminase 1; dsRNA adenosine deaminase; human dsRNA adenosine deaminase DRADA2; adenosine deaminase, RNA-specific, B1 (RED1 homolog rat); adenosine deaminase, RNA-specific, B1 (homolog of rat RED1)
• UniProt Protein Name: RNA editing deaminase 1
• Protein Family: Double-stranded RNA-specific editase
• UniProt Gene Name: hRED1
• UniProt Entry Name: O43263_HUMAN

NCBI Description

This gene encodes the enzyme responsible for pre-mRNA editing of the glutamate receptor subunit B by site-specific deamination of adenosines. Studies in rat found that this enzyme acted on its own pre-mRNA molecules to convert an AA dinucleotide to an AI dinucleotide which resulted in a new splice site. Alternative splicing of this gene results in several transcript variants, some of which have been characterized by the presence or absence of an ALU cassette insert and a short or long C-terminal region. [provided by RefSeq]