Mouse anti-Human Acylation Stimulating Protein Monoclonal Antibody | anti-GHRL antibody

Acylation Stimulating Protein (ASP)

Cat. Num. AAA600639

• Gene Names: GHRL; MTLRP
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Acylation Stimulating Protein; Monoclonal Antibody; Acylation Stimulating Protein (ASP); Anti -Acylation Stimulating Protein (ASP); anti-GHRL antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 5i137
• Specificity: Recognizes human Acylation Stimulating Protein (ASP).
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a lyophilized powder from 0.05M Phosphate buffer, 0.1M sodium chloride, pH 7.2. No Preservative added. Reconstitute in 100ul of sterile dH2O.

• Applicable Applications for anti-GHRL antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.
• Immunogen: Human peptide with amino acid sequence RASHLGLA (which is located in C-terminal part of the human ASP).
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-GHRL antibody

Acylation Stimulating Protein (ASP, also known as C3a desArg) is one of the activation fragments formed from the activation of a complement cascade. ASP is produced through a process involving three proteins: C3, factor B and adipsin, which are secreted by adipocytes. Interactions of C3 with factor B and with adipsin result in the production of C3a followed by desargination of the carboxyl terminus to generate ASP (C3a desArg). Human ASP contains 77 amino acids with 6 cysteins involved in disulfide bridges between residues 22-49, 23-56 and 36-57. ASP is a highly cationic molecule containing no carbohydrate. ASP has a primary role in the regulation of lipid metabolism in adipocytes, where it stimulates glucose uptake, increases the activity of diacylglycerol acyltransferase, and inhibits hormone-sensitive lipase activity. In cellular studies, ASP increases fat storage through increased triglyceride synthesis and decreased intracellular lipolysis. In animal models, ASP-deficient mice demonstrate reduced body weight, reduced leptin and reduced adipose tissue mass. ASP deficiency also results in protection against development of obesity. In humans, a number of studies have shown the relationship between ASP, obesity, diabetes and dyslipidemia. It was reported that the concentration of circulating ASP is positively related to body adiposity and decreases after weight loss. Because ASP enhances triglyceride storage, while interfering with ASP production reduces body fat and protects against diet-induced obesity and insulin resistance, reduction of the production of ASP and ASP receptor antagonists represents a potential approach for treating obesity and type 2 diabetes.

Product Categories/Family for anti-GHRL antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 17865471
• NCBI GeneID: 51738
• UniProt Accession #: Q9UBU3; Q86YP8; Q8TAT9; Q9H3R3; A8CF34; A8CF38; A8CF42; A8DN29; A8DN30
• Molecular Weight: 12,911 Da
• NCBI Official Full Name: Appetite-regulating hormone
• NCBI Official Synonym Full Names: ghrelin/obestatin prepropeptide
• NCBI Official Symbol: GHRL
• NCBI Official Synonym Symbols: MTLRP
• NCBI Protein Information: appetite-regulating hormone; motilin-related peptide; growth hormone secretagogue; ghrelin/obestatin preprohormone; growth hormone-releasing peptide; prepro-appetite regulatory hormone; ghrelin, growth hormone secretagogue receptor ligand
• UniProt Protein Name: Appetite-regulating hormone
• Protein Family: Appetite-regulating hormone
• UniProt Gene Name: GHRL
• UniProt Synonym Gene Names: MTLRP; Ghrelin
• UniProt Entry Name: GHRL_HUMAN

NCBI Description

This gene encodes ghrelin-obestatin preproprotein, which generates ghrelin and obestatin. Ghrelin is an endogenous ligand for the growth hormone secretagogue receptor and is involved in regulating growth hormone release. Obestatin was initially reported to be an endogenous ligand for the orphan G protein-coupled receptor GPR39 and was involved in satiety and decreased food intake; however, these findings are controversial. Recent reports show that obestatin is involved in inhibiting thirst and anxiety, improving memory, regulating sleep, affecting cell proliferation, and increasing the secretion of pancreatic juice enzymes. Alternative promoters and alternative splicing result in multiple transcript variants, some of which encode different protein isoforms and some of which do not encode a protein but may regulate the ghrelin-obestatin preproprotein expression. In addition, antisense transcripts for this gene have been identified and may also function in regulation of the ghrelin-obestatin preproprotein expression. [provided by RefSeq, Oct 2008]

Uniprot Description

ghrelin: a hormone that binds to the growth hormone secretagogue receptor type 1 (GHSR). Secreted by the stomach

Protein type: Secreted; Cell cycle regulation; Secreted, signal peptide; Cell development/differentiation; Apoptosis

Chromosomal Location of Human Ortholog: 3p26-p25

Cellular Component: extracellular space; axon; endoplasmic reticulum lumen; extracellular region

Molecular Function: growth hormone-releasing hormone activity; G-protein-coupled receptor binding; ghrelin receptor binding; protein tyrosine kinase activator activity

Biological Process: cortisol secretion; negative regulation of circadian sleep/wake cycle, REM sleep; positive regulation of cortisol secretion; hormone-mediated signaling; activation of MAPK activity; response to hormone stimulus; negative regulation of interleukin-6 biosynthetic process; decidualization; positive regulation of multicellular organism growth; negative regulation of insulin secretion; positive regulation of adrenocorticotropic hormone secretion; growth hormone secretion; elevation of cytosolic calcium ion concentration; negative regulation of locomotion; positive regulation of appetite; dendrite development; negative regulation of tumor necrosis factor biosynthetic process; positive regulation of circadian sleep/wake cycle, non-REM sleep; regulation of response to food; gastric acid secretion; glucose metabolic process; positive regulation of insulin secretion; adult feeding behavior; negative regulation of interleukin-1 beta production; regulation of cell proliferation; positive regulation of growth hormone secretion; positive regulation of synaptogenesis; G-protein coupled receptor protein signaling pathway; negative regulation of angiogenesis; cellular protein metabolic process; response to estrogen stimulus; negative regulation of inflammatory response; cartilage development; actin polymerization and/or depolymerization; negative regulation of endothelial cell proliferation; regulation of excitatory postsynaptic membrane potential; negative regulation of apoptosis

Disease: Obesity