Mouse Acyl-Coenzyme A Thioesterase 11 Monoclonal Antibody | anti-ACOT11 antibody
Mouse Anti Human Acyl-Coenzyme A Thioesterase 11
Gene Names
ACOT11; BFIT; THEA; THEM1; STARD14
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
ACOT11 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Synonyms
Acyl-Coenzyme A Thioesterase 11; Monoclonal Antibody; Mouse Anti Human Acyl-Coenzyme A Thioesterase 11; ACOT11 Antibody; Mouse Anti Human; Acyl-coenzyme A thioesterase 11; Acyl-CoA thioesterase 11; Acyl-CoA thioester hydrolase 11; Brown fat-inducible thioesterase; BFIT; Adipose-associated thioesterase; ACOT11; KIAA0707; THEA; BFIT1; BFIT2; THEM1; STARD14; anti-ACOT11 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2b heavy chain and kappa light chain
Clone Number
PJ4B2AT
Purity/Purification
ACOT11 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Form/Format
1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.
Sequence Length
607
Applicable Applications for anti-ACOT11 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
ACOT11 antibody has been tested by ELISA, Western blot and immunohistochemistry analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot is 1:500 ~ 1:2,000 and immunohistochemistry analysis is 1:50~100. Recommended starting dilution for Western blot is 1:1,000 and Immunohistochemistry is 1:100.
Immunogen
Anti-human ACOT11 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human ACOT11 amino acids 19-250 purified from E Coli.
Preparation and Storage
For periods up to 1 month store at 4 degree C, for longer periods of time, store at -20 degree C. Prevent freeze thaw cycles.
12 months at -20 degree C. 1 month at 4 degree C.
12 months at -20 degree C. 1 month at 4 degree C.
Related Product Information for anti-ACOT11 antibody
Introduction: ACOT11 is a protein with acyl-CoA thioesterase activity towards medium (C12) and long-chain (C18) fatty acyl-CoA substrates. Expression of a similar murine protein in brown adipose tissue is induced by cold exposure and repressed by warmth. Expression of the mouse ACOT11 has been linked to obesity, with higher expression found in obesity-resistant mice compared with obesity-prone mice.
Product Categories/Family for anti-ACOT11 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
67,152 Da
NCBI Official Full Name
acyl-coenzyme A thioesterase 11 isoform 1
NCBI Official Synonym Full Names
acyl-CoA thioesterase 11
NCBI Official Symbol
ACOT11
NCBI Official Synonym Symbols
BFIT; THEA; THEM1; STARD14
NCBI Protein Information
acyl-coenzyme A thioesterase 11; START domain containing 14; StAR-related lipid transfer (START) domain containing 14; acyl-CoA thioester hydrolase 11; adipose-associated thioesterase; brown fat-inducible thioesterase; thioesterase superfamily member 1; thioesterase, adipose associated
UniProt Protein Name
Acyl-coenzyme A thioesterase 11
Protein Family
UniProt Gene Name
ACOT11
UniProt Synonym Gene Names
BFIT; KIAA0707; THEA; Acyl-CoA thioesterase 11; BFIT
UniProt Entry Name
ACO11_HUMAN
NCBI Description
This gene encodes a member of the acyl-CoA thioesterase family which catalyse the conversion of activated fatty acids to the corresponding non-esterified fatty acid and coenzyme A. Expression of a mouse homolog in brown adipose tissue is induced by low temperatures and repressed by warm temperatures. Higher levels of expression of the mouse homolog has been found in obesity-resistant mice compared with obesity-prone mice, suggesting a role of acyl-CoA thioesterase 11 in obesity. Alternative splicing results in transcript variants. [provided by RefSeq, Nov 2010]
Uniprot Description
ACOT11: Has acyl-CoA thioesterase activity towards medium (C12) and long-chain (C18) fatty acyl-CoA substrates. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Hydrolase; EC 3.1.2.-
Chromosomal Location of Human Ortholog: 1p32.3
Cellular Component: cytoplasm
Molecular Function: acyl-CoA hydrolase activity; lipid binding
Biological Process: response to temperature stimulus; response to cold; fatty acid metabolic process
Research Articles on ACOT11
Similar Products
Product Notes
The ACOT11 acot11 (Catalog #AAA140211) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Acyl-Coenzyme A Thioesterase 11 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). ACOT11 antibody has been tested by ELISA, Western blot and immunohistochemistry analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot is 1:500 ~ 1:2,000 and immunohistochemistry analysis is 1:50~100. Recommended starting dilution for Western blot is 1:1,000 and Immunohistochemistry is 1:100. Researchers should empirically determine the suitability of the ACOT11 acot11 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Acyl-Coenzyme A Thioesterase 11, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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