Goat FMR1 / FMRP Polyclonal Antibody | anti-FMR1 antibody

Goat Polyclonal to Human FMR1 / FMRP

Cat. Num. AAA244746

• Gene Names: FMR1; POF; FMRP; POF1; FRAXA
• Reactivity: Bat, Gibbon, Dog, Gorilla, Hamster, Human, Monkey, Mouse, Orangutan, Pig, Rabbit, Rat; Predicted Reactivity: Bovine (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: FMR1 / FMRP; Polyclonal Antibody; Goat Polyclonal to Human FMR1 / FMRP; Anti-FMR1 / FMRP Antibody IHC-plus; FMR1; FMRP; POF; Protein FMR-1; Premature ovarian failure 1; Fragile X mental retardation 1; FRAXA; POF1; Human FMR1; anti-FMR1 antibody

• Host: Goat
• Reactivity: Bat, Gibbon, Dog, Gorilla, Hamster, Human, Monkey, Mouse, Orangutan, Pig, Rabbit, Rat; Predicted Reactivity: Bovine (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human FMR1.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 632

• Applicable Applications for anti-FMR1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:32000), IHC-P (2.5 ug/ml), WB (0.3 - 1 ug/ml); Usage: Approx 70Da band observed in lysates of cell lines HeLa and Jurkat (calculated MW of 71.2kDa according to NP_002015.1).
• Target Species: Human
• Non Reactive Species: Chicken
• Immunogen Description: Synthetic peptide C-NPNKPATKDTFHKIK from an internal region of human FMR1 (NP_002015.1).
• Immunogen Type: Synthetic peptide
• Immunogen: FMR1 / FMRP antibody was raised against synthetic peptide C-NPNKPATKDTFHKIK from an internal region of human FMR1 (NP_002015.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus (100%); Bovine (93%); Xenopus (80%).
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-FMR1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-FMR1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.)

Western Blot (WB)

(Antibody (0.3 ug/ml) staining of HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 4503765
• NCBI GeneID: 2332
• NCBI Accession #: NP_002015.1
• NCBI GenBank Nucleotide #: NM_002024.5
• UniProt Accession #: Q06787; Q16578; Q5PQZ6; Q99054; A6NNH4; D3DWT0; D3DWT1; D3DWT2; G8JL90
• Molecular Weight: 68,966 Da
• NCBI Official Full Name: fragile X mental retardation protein 1 isoform ISO1
• NCBI Official Synonym Full Names: fragile X mental retardation 1
• NCBI Official Symbol: FMR1
• NCBI Official Synonym Symbols: POF; FMRP; POF1; FRAXA
• NCBI Protein Information: fragile X mental retardation protein 1
• UniProt Protein Name: Fragile X mental retardation protein 1
• Protein Family: Mat- sexual cell fertilization-promoting factor
• UniProt Gene Name: FMR1
• UniProt Synonym Gene Names: FMRP; Protein FMR-1
• UniProt Entry Name: FMR1_HUMAN

NCBI Description

The protein encoded by this gene binds RNA and is associated with polysomes. The encoded protein may be involved in mRNA trafficking from the nucleus to the cytoplasm. A trinucleotide repeat (CGG) in the 5' UTR is normally found at 6-53 copies, but an expansion to 55-230 repeats is the cause of fragile X syndrome. Expansion of the trinucleotide repeat may also cause one form of premature ovarian failure (POF1). Multiple alternatively spliced transcript variants that encode different protein isoforms and which are located in different cellular locations have been described for this gene. [provided by RefSeq, May 2010]

Uniprot Description

FMR1: Translation repressor. Component of the CYFIP1-EIF4E- FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates translation repression. RNA- binding protein that plays a role in intracellular RNA transport and in the regulation of translation of target mRNAs. Associated with polysomes. May play a role in the transport of mRNA from the nucleus to the cytoplasm. Binds strongly to poly(G), binds moderately to poly(U) but shows very little binding to poly(A) or poly(C). Component of the CYFIP1-EIF4E-FMR1 complex which is composed of CYFIP, EIF4E and FMR1. Interacts with CYFIP1 and CYFIP2. The interaction with brain cytoplasmic RNA 1 (BC1) increases binding affinity for the CYFIP1-EIF4E complex in the brain. Homooligomer. Found in a RNP granule complex with IGF2BP1. Directly interacts with SMN and TDRD3. Interacts with the SMN core complex that contains SMN1, GEMIN2/SIP1, DDX20/GEMIN3, GEMIN4, GEMIN5, GEMIN6, GEMIN7, GEMIN8 and STRAP/UNRIP. Interacts with FXR1, FXR2, IGF2BP1, NUFIP1, NUFIP2, MCRS1 and RANBP9. Highest levels found in neurons, brain, testis, placenta and lymphocytes. Also expressed in epithelial tissues and at very low levels in glial cells. Belongs to the FMR1 family. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; Nucleolus; Translation

Chromosomal Location of Human Ortholog: Xq27.3

Cellular Component: nucleoplasm; microtubule; mRNA cap complex; polysomal ribosome; membrane; stress granule; cytoplasm; nucleolus; dendritic spine; synapse; dendritic shaft

Molecular Function: mRNA binding; protein binding; RNA binding; microtubule binding

Biological Process: negative regulation of translational initiation; mRNA transport; central nervous system development; negative regulation of translation

Disease: Fragile X Mental Retardation Syndrome; Fragile X Tremor/ataxia Syndrome; Premature Ovarian Failure 1