Mouse anti-Human ACY1 / Aminoacylase 1 Monoclonal Antibody | anti-ACY1 antibody

Mouse Monoclonal [clone 4F1-B7] (IgG1,k) to Human ACY1 / Aminoacylase 1

Cat. Num. AAA245765

• Gene Names: ACY1; ACY-1; ACY1D; HEL-S-5
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, Immunoprecipitation, ELISA
• Purity: Protein A Purified
• Synonyms: ACY1 / Aminoacylase 1; Monoclonal Antibody; Mouse Monoclonal [clone 4F1-B7] (IgG1; k) to Human ACY1 / Aminoacylase 1; Anti-ACY1 / Aminoacylase 1 Antibody (clone 4F1-B7) IHC-plus; Aminoacylase-1; ACY-1; ACY1D; Acylase; Acylase I; Aminoacylase 1; Aminoacylase; Aminoacylase I; Human ACY1; anti-ACY1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 4F1-B7
• Specificity: Human Aminoacylase-1
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.2. Sourced in Ascites.
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 408

• Applicable Applications for anti-ACY1 antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), Immunoprecipitation (IP), ELISA (EIA)
• Application Notes: ELISA, IF (10 ug/ml), IHC-P (5 ug/ml), IP, WB (1:500 - 1:1000); Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Transfected lysate, Recombinant protein.
• Target Species: Human
• Immunogen Description: ACY1 (AAH00545, 1 a.a. ~ 409 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen: ACY1 / Aminoacylase 1 antibody was raised against aCY1 (AAH00545, 1 a.a. ~ 409 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-ACY1 / Aminoacylase-1 antibody IHC of human kidney, tubules. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to ACY1 on HeLa cell. [antibody concentration 10 ug/ml])

Western Blot (WB)

(Western blot of ACY1 expression in transfected 293T cell line by ACY1 monoclonal antibody (M01), clone 4F1-B7.)

Western Blot (WB)

(Western blot of ACY1 expression in K-562 by ACY1 monoclonal antibody (M01), clone 4F1-B7.)

Immunoprecipitation (IP)

(Immunoprecipitation of ACY1 transfected lysate using anti-ACY1 monoclonal antibody and Protein A Magnetic Bead.)

Product Categories/Family for anti-ACY1 antibody

Family: Protease; Subfamily: Metallopeptidase M20

NCBI and Uniprot Product Information

• NCBI GI #: 4501901
• NCBI GeneID: 95
• NCBI Accession #: NP_000657.1
• NCBI GenBank Nucleotide #: NM_000666.2
• UniProt Accession #: Q03154; C9J6I6; C9J9D8; C9JWD4
• Molecular Weight: 42,195 Da
• NCBI Official Full Name: aminoacylase-1 isoform a
• NCBI Official Synonym Full Names: aminoacylase 1
• NCBI Official Symbol: ACY1
• NCBI Official Synonym Symbols: ACY-1; ACY1D; HEL-S-5
• NCBI Protein Information: aminoacylase-1; epididymis secretory protein Li 5; N-acyl-L-amino-acid amidohydrolase
• UniProt Protein Name: Aminoacylase-1
• Protein Family: Aminoacylase
• UniProt Gene Name: ACY1
• UniProt Synonym Gene Names: ACY-1
• UniProt Entry Name: ACY1_HUMAN

NCBI Description

This gene encodes a cytosolic, homodimeric, zinc-binding enzyme that catalyzes the hydrolysis of acylated L-amino acids to L-amino acids and an acyl group, and has been postulated to function in the catabolism and salvage of acylated amino acids. This gene is located on chromosome 3p21.1, a region reduced to homozygosity in small-cell lung cancer (SCLC), and its expression has been reported to be reduced or undetectable in SCLC cell lines and tumors. The amino acid sequence of human aminoacylase-1 is highly homologous to the porcine counterpart, and this enzyme is the first member of a new family of zinc-binding enzymes. Mutations in this gene cause aminoacylase-1 deficiency, a metabolic disorder characterized by central nervous system defects and increased urinary excretion of N-acetylated amino acids. Alternative splicing of this gene results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream ABHD14A (abhydrolase domain containing 14A) gene, as represented in GeneID:100526760. A related pseudogene has been identified on chromosome 18. [provided by RefSeq, Nov 2010]

Uniprot Description

ACY1: Involved in the hydrolysis of N-acylated or N-acetylated amino acids (except L-aspartate). Defects in ACY1 are the cause of aminoacylase-1 deficiency (ACY1D). ACY1D results in a metabolic disorder manifesting with encephalopathy, unspecific psychomotor delay, psychomotor delay with atrophy of the vermis and syringomyelia, marked muscular hypotonia or normal clinical features. Epileptic seizures are a frequent feature. All affected individuals exhibit markedly increased urinary excretion of several N-acetylated amino acids. Belongs to the peptidase M20A family.

Protein type: Amino Acid Metabolism - arginine and proline; EC 3.5.1.14; Hydrolase

Chromosomal Location of Human Ortholog: 3p21.1

Cellular Component: cytosol

Molecular Function: metallopeptidase activity; metal ion binding; aminoacylase activity

Biological Process: amino acid metabolic process; xenobiotic metabolic process; proteolysis

Disease: Aminoacylase 1 Deficiency