Mouse anti-Human Activated C1s Monoclonal Antibody
Activated C1s, Human, mAb M241
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot
Synonyms
Activated C1s; Monoclonal Antibody; Human; mAb M241; anti-Activated C1s antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG2a
Form/Format
1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS containing 0.1% bovine serum albumin.
For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
Applicable Applications for anti-Activated C1s antibody
F, Flow Cytometry (FC/FACS), FS, Immuno Assay (IA), Immunoprecipitaion (IP), P, Western Blot (WB)
Application Notes
Application Use: For immunohistology, flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For inhibition of biological activity dilutions have to be made according to the amounts C1s to be inactivated.
Preparation and Storage
Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.
Related Product Information for anti-Activated C1s antibody
Product Description: Monoclonal antibody M241 reacts with an epitope on human C protein activated C1s, a subcomponent of the first component of C (C1). Activated C1s is a glycosylated single-polypeptide zymogen of 85 kD. Activation of the proenzyme C1s occurs through cleavage by the active form of C1r. The activated protease, activated C1s, consists of a disulfide-linked H chain and a L chain. Activated C1s is a serine protease and its catalytic site is located in the L chain. Activation of the classical C pathway is triggered by activated C1s which cleaves C4 and C2 to form the C3 convertase, C4bC2a. The epitope recognised by the antibody M241 is domain IV and/or V of the gamma-domain of activated C1s. Monoclonal antibody M241 blocks C4 activation and C4 binding to activated C1s. The antibody binds specifically to the active centre of C1s.
References
1. Matsumoto, M et al; Probing the C4-binding site on C1s with monoclonal antibodies, evidence for a C4/C4b-binding site on the gamma-domain. J Immunol 1989, 8: 2743 2. Nakagawa, K et al; Complement C1s activation in degenerating articular cartilage of rheumatoid arthritis patients: immunohistochemical studies with an active form specific antibody. Ann Rheum Dis 1999, 58: 175
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Product Notes
The Activated C1s (Catalog #AAA584453) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Activated C1s, Human, mAb M241 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Activated C1s can be used in a range of immunoassay formats including, but not limited to, F, Flow Cytometry (FC/FACS), FS, Immuno Assay (IA), Immunoprecipitaion (IP), P, Western Blot (WB). Application Use: For immunohistology, flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For inhibition of biological activity dilutions have to be made according to the amounts C1s to be inactivated. Researchers should empirically determine the suitability of the Activated C1s for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Activated C1s, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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