Mouse anti-Human Actin, alpha Monoclonal Antibody | anti-ACTA1 antibody

Actin, alpha (ACTA1, ACTA, skeletal muscle, Alpha-actin-1) (Azide free) (HRP)

Cat. Num. AAA6121437

• Gene Names: ACTA1; ACTA; ASMA; CFTD; MPFD; NEM1; NEM2; NEM3; CFTD1; CFTDM
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography
• Synonyms: Actin; alpha; Monoclonal Antibody; alpha (ACTA1; ACTA; skeletal muscle; Alpha-actin-1) (Azide free) (HRP); ACTA1; alpha skeletal muscle; Alpha-actin-1; anti-ACTA1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 337CT30.10.1
• Specificity: Recognizes human Actin, alpha.
• Purity/Purification: Purified by Protein G Affinity Chromatography
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).

• Applicable Applications for anti-ACTA1 antibody: ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human Actin, alpha. Species Sequence Homology: bovine, chicken, mouse, procine, rabbit and rat
• Conjugate: HRP
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in CEM cell line lysates (35ug/lane) using. This demonstrates that detected the ACTA1 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma using, followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of for immunohistochemistry.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle using, followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of for immunohistochemistry.)

Testing Data

Product Categories/Family for anti-ACTA1 antibody

Antibodies; Disease Markers

References

1. Kim, E.Y., et al. Am. J. Physiol. Renal Physiol. 299 (3), F594-F604 (2010) 2. Haigh, S.E., et al. Neuromuscul. Disord. 20(6):363-374(2010) 3. Yu, G., et al. J Clin Neurosci 17(6):766-769(2010) 4. Yu, C.H., et al. PLoS ONE 5 (7), E11878 (2010) 5. Licastro, F., et al. Curr. Pharm. Des. 16(7):783-788(2010)

NCBI and Uniprot Product Information

• NCBI GI #: 4501881
• NCBI GeneID: 58
• NCBI Accession #: NP_001091.1
• NCBI GenBank Nucleotide #: NM_001100.3
• UniProt Accession #: P68133; P02568; P99020; Q5T8M9
• Molecular Weight: 42,051 Da
• NCBI Official Full Name: actin, alpha skeletal muscle
• NCBI Official Synonym Full Names: actin, alpha 1, skeletal muscle
• NCBI Official Symbol: ACTA1
• NCBI Official Synonym Symbols: ACTA; ASMA; CFTD; MPFD; NEM1; NEM2; NEM3; CFTD1; CFTDM
• NCBI Protein Information: actin, alpha skeletal muscle; nemaline myopathy type 3
• UniProt Protein Name: Actin, alpha skeletal muscle
• Protein Family: Actin
• UniProt Gene Name: ACTA1
• UniProt Synonym Gene Names: ACTA
• UniProt Entry Name: ACTS_HUMAN

NCBI Description

The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects. [provided by RefSeq, Jul 2008]

Uniprot Description

ACTA1: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Interacts with TTID. Interacts (via its C-terminus) with USP25; the interaction occurs for all USP25 isoforms but is strongest for isoform USP25m in muscle differentiating cells. Belongs to the actin family.

Protein type: Cell development/differentiation; Motility/polarity/chemotaxis; Contractile; Cytoskeletal

Chromosomal Location of Human Ortholog: 1q42.13

Cellular Component: extracellular space; sarcomere; stress fiber; actin filament; cytosol; striated muscle thin filament; actin cytoskeleton

Molecular Function: protein binding; myosin binding; structural constituent of cytoskeleton; ADP binding; ATP binding

Biological Process: skeletal muscle fiber adaptation; muscle contraction; muscle thin filament assembly; response to steroid hormone stimulus; response to mechanical stimulus; response to lithium ion; response to extracellular stimulus; skeletal muscle fiber development; cell growth; muscle filament sliding

Disease: Nemaline Myopathy 3; Myopathy, Congenital, With Fiber-type Disproportion