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Western Blot (Western Blot analysis using ACSS2 antibodyWestern Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant ACSS2 protein (Right) or empty vector (Left) detected with ACSS2 antibody)

Mouse ACSS2 Monoclonal Antibody | anti-ACSS2 antibody

ACSS2 antibody

Gene Names
ACSS2; ACS; ACSA; ACAS2; ACECS; dJ1161H23.1
Applications
Western Blot
Purity
ACSS2 antibody was purified by affinity chromatography.
Synonyms
ACSS2; Monoclonal Antibody; ACSS2 antibody; Monoclonal ACSS2; Anti-ACSS2; Acyl-CoA synthetase short-chain family member 2; ACS; ACSA; Acetate CoA ligase; Acyl-activating enzyme; ACAS2; Acetate thiokinase; Acetate-CoA ligase; Acetyl-CoA synthetase; ACSS 2; ACSS-2; anti-ACSS2 antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
3G3
Purity/Purification
ACSS2 antibody was purified by affinity chromatography.
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration
500 ug-1 mg/ml (varies by lot)
Sequence Length
701
Applicable Applications for anti-ACSS2 antibody
Western Blot (WB)
Application Notes
WB: 1:2000
Biological Significance
ACSS2 is a cytosolic enzyme that catalyzes the activation of acetate for use in lipid synthesis and energy generation. The protein acts as a monomer and produces acetyl-CoA from acetate in a reaction that requires ATP.
Cross-Reactivity
Human
Immunogen
ACSS2 antibody was raised in mouse using a full length recombinant protein of human ACSS2 (NP_061147) produced in HEK293T cells, as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Western Blot

(Western Blot analysis using ACSS2 antibodyWestern Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant ACSS2 protein (Right) or empty vector (Left) detected with ACSS2 antibody)

Western Blot (Western Blot analysis using ACSS2 antibodyWestern Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant ACSS2 protein (Right) or empty vector (Left) detected with ACSS2 antibody)
Related Product Information for anti-ACSS2 antibody
Mouse monoclonal ACSS2 antibody
Product Categories/Family for anti-ACSS2 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
80,087 Da
NCBI Official Full Name
acetyl-coenzyme A synthetase, cytoplasmic isoform 1
NCBI Official Synonym Full Names
acyl-CoA synthetase short-chain family member 2
NCBI Official Symbol
ACSS2
NCBI Official Synonym Symbols
ACS; ACSA; ACAS2; ACECS; dJ1161H23.1
NCBI Protein Information
acetyl-coenzyme A synthetase, cytoplasmic
UniProt Protein Name
Acetyl-coenzyme A synthetase, cytoplasmic
UniProt Gene Name
ACSS2
UniProt Synonym Gene Names
ACAS2; ACS; AceCS
UniProt Entry Name
ACSA_HUMAN

NCBI Description

This gene encodes a cytosolic enzyme that catalyzes the activation of acetate for use in lipid synthesis and energy generation. The protein acts as a monomer and produces acetyl-CoA from acetate in a reaction that requires ATP. Expression of this gene is regulated by sterol regulatory element-binding proteins, transcription factors that activate genes required for the synthesis of cholesterol and unsaturated fatty acids. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2009]

Uniprot Description

ACSS2: Activates acetate so that it can be used for lipid synthesis or for energy generation. Monomer. Belongs to the ATP-dependent AMP-binding enzyme family.

Protein type: EC 6.2.1.1; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - pyruvate; Carbohydrate Metabolism - propanoate; Ligase

Chromosomal Location of Human Ortholog: 20q11.22

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; cytoplasm; cytosol

Molecular Function: acetate-CoA ligase activity; ATP binding; AMP binding

Biological Process: acetyl-CoA biosynthetic process from acetate; xenobiotic metabolic process; lipid biosynthetic process; ethanol oxidation; propionate biosynthetic process; acetate biosynthetic process

Research Articles on ACSS2

Similar Products

Product Notes

The ACSS2 acss2 (Catalog #AAA831926) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's ACSS2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:2000. Researchers should empirically determine the suitability of the ACSS2 acss2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ACSS2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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