Western Blot (WB)
(The HepG2 cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACSF2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Mouse anti-Human ACSF2 Monoclonal Antibody | anti-ACSF2 antibody
ACSF2 antibody
Gene Names
ACSF2; ACSMW; AVYV493
Reactivity
Human
Applications
ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
Purity
By protein-A affinity chromatography
Synonyms
ACSF2; Monoclonal Antibody; ACSF2 antibody; Acyl-CoA synthetase family member 2 isoform 2; ACSMW; AVYV493; anti-ACSF2 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
AT18B4
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
615
Applicable Applications for anti-ACSF2 antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Antigen Species
Human
Immunogen
Recombinant human ACSF2 (42-615aa) purified from E. coli
Clone Note
Anti-human ACSF2 mAb, clone AT18B4, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human ACSF2 protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Western Blot (WB)
(The HepG2 cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACSF2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Western Blot (WB)
(The HepG2 cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACSF2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Immunofluorescence (IF)
(ICC/IF analysis of ACSF2 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ACSF2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green))
Immunofluorescence (IF)
(ICC/IF analysis of ACSF2 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ACSF2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green))
Related Product Information for anti-ACSF2 antibody
ACSF2 also known as Acyl-CoA synthetase family member 2. Acyl-CoA synthetases are a family of enzymes that catalyze the thioesterification of fatty acids with coenzymeA to form activated intermediates, which play a fundamental role in lipid metabolism and homeostasis of lipid-related processes. ACSF2 are required for complex lipid synthesis, energy production via beta-oxidation, protein acylation and fatty-acid dependent transcriptional regulation. ACSF2 are also necessary for fatty acid import into cells by the process of vectorial acylation.
Product Categories/Family for anti-ACSF2 antibody
References
Black PN., et al. (2007) Biochim Biophys Acta. 1771 (9): 1246-53.; Watkins P.A., et al. (2007) J Lipid Res. 48 (12): 2736-50.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
50,880 Da
NCBI Official Full Name
acyl-CoA synthetase family member 2, mitochondrial isoform 2
NCBI Official Synonym Full Names
acyl-CoA synthetase family member 2
NCBI Official Symbol
ACSF2
NCBI Official Synonym Symbols
ACSMW; AVYV493
NCBI Protein Information
acyl-CoA synthetase family member 2, mitochondrial
UniProt Protein Name
Acyl-CoA synthetase family member 2, mitochondrial
Protein Family
UniProt Gene Name
ACSF2
Uniprot Description
Acyl-CoA synthases catalyze the initial reaction in fatty acid metabolism, by forming a thioester with CoA. Has some preference toward medium-chain substrates. Plays a role in adipocyte differentiation.
Research Articles on ACSF2
Similar Products
Product Notes
The ACSF2 acsf2 (Catalog #AAA200470) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ACSF2 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ACSF2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF). Researchers should empirically determine the suitability of the ACSF2 acsf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ACSF2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
