Mouse anti-Human ACOT7 Monoclonal Antibody | anti-ACOT7 antibody

ACOT7 (BACH, Cytosolic Acyl Coenzyme A Thioester Hydrolase, Long Chain Acyl-CoA Thioester Hydrolase, Acyl-CoA Thioesterase 7, CTE-IIa, CTE-II, Brain Acyl-CoA Hydrolase, MGC1126, RP1-120G22.10) (Biotin)

Cat. Num. AAA6140446

• Gene Names: ACOT7; ACT; ACH1; BACH; LACH; LACH1; hBACH; CTE-II
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ACOT7; Monoclonal Antibody; ACOT7 (BACH; Cytosolic Acyl Coenzyme A Thioester Hydrolase; Long Chain Acyl-CoA Thioester Hydrolase; Acyl-CoA Thioesterase 7; CTE-IIa; CTE-II; Brain Acyl-CoA Hydrolase; MGC1126; RP1-120G22.10) (Biotin); anti-ACOT7 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 1E7
• Specificity: Recognizes human ACOT7.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-ACOT7 antibody: ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IHC-P: 3ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Full-length recombinant corresponding to aa1-339 from ACOT7 (AAH17365) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MSGPDVETPSAIQICRIMRPDDANVAGNVHGGTILKMIEEAGAIISTRHCNSQNGERCVAALARVERTDFLSPMCIGEVAHVSAEITYTSKHSVEVQVNVMSENILTGAKKLTNKATLWYVPLSLKNVDKVLEVPPVVYSRQEQEEEGRKRYEAQKLERMETKWRNGDIVQPVLNPEPNTVSYSQSSLIHLVGPSDCTLHGFVHGGVTMKLMDEVAGIVAARHCKTNIVTASVDAINFHDKIRKGCVITISGRMT
• Conjugate: Biotin
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (63.29kD).)

Western Blot (WB)

(ACOT7 monoclonal antibody Western Blot analysis of ACOT7 expression in Jurkat.)

Western Blot (WB)

(Western Blot analysis of ACOT7 expression in transfected 293T cell line by ACOT7 monoclonal antibody. Lane 1: ACOT7 transfected lysate (Predicted MW: 41.8kD). Lane 2: Non-transfected lysate.)

Immunohistochemistry (IHC)

(Immunoperoxidase of monoclonal antibody to ACOT7 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3ug/ml])

Testing Data

(Detection limit for recombinant GST tagged ACOT7 is 0.3ng/ml as a capture antibody.)

Product Categories/Family for anti-ACOT7 antibody

Antibodies; Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 33869295
• NCBI GeneID: 11332
• Molecular Weight: 40,455 Da
• NCBI Official Full Name: Homo sapiens acyl-CoA thioesterase 7, mRNA
• NCBI Official Synonym Full Names: acyl-CoA thioesterase 7
• NCBI Official Symbol: ACOT7
• NCBI Official Synonym Symbols: ACT; ACH1; BACH; LACH; LACH1; hBACH; CTE-II
• NCBI Protein Information: cytosolic acyl coenzyme A thioester hydrolase
• Protein Family: Cytosolic acyl coenzyme A thioester hydrolase

NCBI Description

This gene encodes a member of the acyl coenzyme family. The encoded protein hydrolyzes the CoA thioester of palmitoyl-CoA and other long-chain fatty acids. Decreased expression of this gene may be associated with mesial temporal lobe epilepsy. Alternatively spliced transcript variants encoding distinct isoforms with different subcellular locations have been characterized. [provided by RefSeq, Jul 2008]