Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant ACOT12 protein (Right) or empty vector (Left) detected with ACOT12 antibody)
Mouse ACOT12 Monoclonal Antibody | anti-ACOT12 antibody
ACOT12 antibody
Gene Names
ACOT12; Cach; THEAL; CACH-1; STARD15
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Purity
ACOT12 antibody was purified by affinity chromatography.
Synonyms
ACOT12; Monoclonal Antibody; ACOT12 antibody; Monoclonal ACOT12; Anti-ACOT12; Acyl-Coa Thioesterase 12; CACH-1; Cach; MGC105114; STARD15; THEAL; ACOT 12; ACOT-12; anti-ACOT12 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
1B9
Purity/Purification
ACOT12 antibody was purified by affinity chromatography.
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration
500 ug-1 mg/ml (varies by lot)
Sequence Length
555
Applicable Applications for anti-ACOT12 antibody
Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
FC: 1:100
IHC: 1:150
WB: 1:500-2000
IHC: 1:150
WB: 1:500-2000
Biological Significance
ACOT12 contains 2 acyl coenzyme A hydrolase domains and 1 START domain. It hydrolyzes acetyl-CoA to acetate and CoA.
Cross-Reactivity
Human, Monkey
Immunogen
ACOT12 antibody was raised in mouse using a full length recombinant protein of human ACOT12 (NP_570123) produced in HEK293T cells, as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant ACOT12 protein (Right) or empty vector (Left) detected with ACOT12 antibody)
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant ACOT12 protein (Right) or empty vector (Left) detected with ACOT12 antibody)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of ACOT12 protein in paraffin embedded Human Kidney tissue using ACOT12 antibody)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of ACOT12 protein in paraffin embedded Human Kidney tissue using ACOT12 antibody)
Related Product Information for anti-ACOT12 antibody
Mouse monoclonal ACOT12 antibody
Product Categories/Family for anti-ACOT12 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
18,327 Da
NCBI Official Full Name
acyl-coenzyme A thioesterase 12
NCBI Official Synonym Full Names
acyl-CoA thioesterase 12
NCBI Official Symbol
ACOT12
NCBI Official Synonym Symbols
Cach; THEAL; CACH-1; STARD15
NCBI Protein Information
acyl-coenzyme A thioesterase 12
UniProt Protein Name
Acyl-coenzyme A thioesterase 12
Protein Family
UniProt Gene Name
ACOT12
UniProt Synonym Gene Names
CACH; CACH1; STARD15; Acyl-CoA thioesterase 12; CACH-1; hCACH-1; StARD15
UniProt Entry Name
ACO12_HUMAN
Uniprot Description
ACOT12: Hydrolyzes acetyl-CoA to acetate and CoA.
Protein type: Carbohydrate Metabolism - pyruvate; Hydrolase; EC 3.1.2.1
Chromosomal Location of Human Ortholog: 5q14.1
Cellular Component: cytosol
Molecular Function: acetyl-CoA hydrolase activity; lipid binding; ATP binding
Biological Process: acyl-CoA metabolic process; acetyl-CoA metabolic process; fatty acid metabolic process; pyruvate metabolic process
Research Articles on ACOT12
Similar Products
Product Notes
The ACOT12 acot12 (Catalog #AAA831236) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's ACOT12 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Western Blot (WB). FC: 1:100 IHC: 1:150 WB: 1:500-2000. Researchers should empirically determine the suitability of the ACOT12 acot12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ACOT12, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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