Mouse anti-E Coli acnB Monoclonal Antibody | anti-acnB antibody
Anti-acnB Antibody, Mouse Monoclonal
Reactivity
E Coli
Applications
ELISA
Purity
Protein A
Synonyms
acnB; Monoclonal Antibody; Anti-acnB Antibody; Mouse Monoclonal; Escherichia coli acnB Antibody; Mouse MAb; Anti-yacI Antibody; Anti-yacJ Antibody; anti-acnB antibody
Host
Mouse
Reactivity
E Coli
Clonality
Monoclonal
Clone Number
02
Specificity
Escherichia coli acnB
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-acnB antibody
ELISA (EIA) (Det)
Application Notes
Antibody's applications have not been validated with corresponding E Coli.
ELISA (Det): This antibody will detect Escherichia coli acnB in ELISA pair set.
In a sandwich ELISA, it can be used as detection antibody when paired with.
ELISA (Det): This antibody will detect Escherichia coli acnB in ELISA pair set.
In a sandwich ELISA, it can be used as detection antibody when paired with.
Immunogen
Recombinant Escherichia coli acnB
Conjugation
Unconjugated
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Escherichia coli acnB. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Related Product Information for anti-acnB antibody
Escherichia coli contains two major aconitases (Acns), AcnA and AcnB. They are distantly related monomeric Fe-S proteins that contain different arrangements of four structural domains. acnA is specifically subject to SoxRS-mediated activation, whereas acnB encodes the major aconitase that is synthesized earlier in the growth cycle than AcnA. It is concluded that AcnB is the major citric acid cycle enzyme. Aconitate hydratase 2 (acnB) catalyzes the isomerization of citrate to isocitrate via cis-aconitate as well as the dehydration of 2-methylisocitrate to cis-2-methylaconitate, thus it functions as the major citric-acid-cycle enzyme during exponential growth. Escherichia coli acnB serves as either an enzymic catalyst or a mRNA-binding post-transcriptional regulator, depending on the status of its iron sulfur cluster. AcnB represents a large, distinct group of Gram-negative bacterial aconitases that have an altered domain organization relative to mitochondrial aconitase and other aconitases.
References
Bradbury AJ. et al., 1996, 142 (Pt 2): 389-400. Cunningham L. et al., 1997, Microbiology. 143 (Pt 12): 3795-805. Jordan PA. et al., 1999, Biochem J. 344 Pt 3: 739-46. Williams CH. et al., 2002, Nat Struct Biol. 9(6): 447-52.
NCBI and Uniprot Product Information
UniProt Accession #
Protein Family
Similar Products
Product Notes
The acnB (Catalog #AAA8106731) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-acnB Antibody, Mouse Monoclonal reacts with E Coli and may cross-react with other species as described in the data sheet. AAA Biotech's acnB can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA) (Det). Antibody's applications have not been validated with corresponding E Coli. ELISA (Det): This antibody will detect Escherichia coli acnB in ELISA pair set. In a sandwich ELISA, it can be used as detection antibody when paired with. Researchers should empirically determine the suitability of the acnB for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "acnB, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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