Mouse anti-Human Abp1 Monoclonal Antibody | anti-Abp1 antibody

Abp1 (C-terminal region) Antibody

Cat. Num. AAA474174

• Gene Names: DBNL; ABP1; HIP55; SH3P7; HIP-55
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunocytochemistry
• Synonyms: Abp1; Monoclonal Antibody; Abp1 (C-terminal region) Antibody; Abp1 (C-terminal region); Abp; anti-Abp1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: M334
• Specificity: This antibody detects a 55kDa* protein corresponding to the molecular mass of Abp1 on SDS-PAGE immunoblots of human Jurkat, Hela, and A431 cells, but does not detect Abp1 in mouse brain.
• Form/Format: Mouse monoclonal, protein A purified antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.

• Applicable Applications for anti-Abp1 antibody: Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC)
• Application Notes: ELISA: 1:2000; ICC: 1:100; WB: 1:1000
• Immunogen: Clone (M334) was generated from a recombinant sequence that included amino acids in the C-terminal region of human Abp1.
• Preparation and Storage: Store at -20 degree C. Stable for 1 year.

Western Blot (WB)

(Western blot of Jurkat (lane 1), HeLa (lane 2), mouse brain (lane 3), and A431 (lane 4). The blot was probed with mouse monoclonal anti-Abp1 (C-terminal region) antibody at 1:500.)

Immunocytochemistry (ICC)

(Immunocytochemical labeling of Abp1 in aldehyde-fixed and NP-40-permeabilized differentiated A431 cells. The cells were labeled with mouse monoclonal anti-Abp1 (AM3341) antibody, then the antibody was detected using appropriate secondary antibody conjugated to DyLight 594. The cells were counterstained with Sytox green to label nuclei.)

Related Product Information for anti-Abp1 antibody

Cellular morphology, adhesion, and motility occur through dynamic reorganization of actin-based superstructures. Actin-binding proteins are critical for regulating actin polymerization and superstructure formation. The Arp2/3 complex is an actin polymerization-inducing complex that includes Arp2, Arp3, p41-Arc, p34-Arc, p21-Arc, p20 Arc, and p16-Arc. Several actin binding proteins, WASP, cortactin, and Abp1, regulate the activity of the Arp2/3 complex. Abp1 (SH3P7, HIP-55) is the mammalian homologue of the yeast Abp1p, which has been shown to bind actin and to have roles in endocytosis. Abp1 contains a C-terminal Src homology 3 domain and an N-terminal actin depolymerization factor (ADF-H/C) domain that is homologous to a similar domain found in the actin severing protein, cofilin. Abp1 can bind Src kinases, hematopoietic progenitor kinase 1 (HPK-1), myosin I heavy chain kinase, and dynamin. Thus, Abp1 may be important for regulation of the actin cytoskeleton through its ability to interact with F-actin and various other cytoskeletal and cell signaling proteins.

References

Kessels, M.M. et al. (2001) J Cell Biol. 153(2):351.
Olazabal, I.M. & Machesky, L.M. (2001) J Cell Biol. 154(4):679.
Larbolette, O. et al. (1999) Mol Cell Biol. 19(2):1539.

NCBI and Uniprot Product Information

• NCBI GI #: 51316115
• NCBI GeneID: 28988
• UniProt Accession #: Q9UJU6; P84070; Q6IAI8; Q96F30; Q96K74; Q9HBN8; Q9NR72; A4D2I9; B4DDP6; B4DEM2; C9J7P1
• Molecular Weight: 430
• NCBI Official Full Name: Drebrin-like protein
• NCBI Official Synonym Full Names: drebrin-like
• NCBI Official Symbol: DBNL
• NCBI Official Synonym Symbols: ABP1; HIP55; SH3P7; HIP-55
• NCBI Protein Information: drebrin-like protein; drebrin-F; cervical SH3P7; SH3 domain-containing protein 7; cervical mucin-associated protein; HPK1-interacting protein of 55 kDa; src homology 3 domain-containing protein HIP-55
• UniProt Protein Name: Drebrin-like protein
• Protein Family: ARS-binding protein
• UniProt Gene Name: DBNL
• UniProt Synonym Gene Names: CMAP; SH3P7; HIP-55
• UniProt Entry Name: DBNL_HUMAN

Uniprot Description

DBNL: an SH3-containing adaptor protein. Interacts with hematopoietic progenitor kinase 1a (HIPK1). It is recruited to glycolipid-enriched microdomains following T cell receptor (TCR) stimulation. Interacts with and is phosphorylated by ZAP-70 after TCR signaling. May regulate JNK activation following TCR signaling, presumably via its interaction with HIPKIt is a member of the drebrin family of proteins that are developmentally regulated in the brain.

Protein type: Actin-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 7p13

Cellular Component: Golgi membrane; ruffle; lamellipodium; dendrite; postsynaptic density; cytoplasm; plasma membrane; clathrin coated vesicle membrane; podosome; cell cortex; cytosol; cell junction

Molecular Function: actin filament binding; protein C-terminus binding; protein domain specific binding; protein binding; enzyme activator activity; actin binding

Biological Process: receptor-mediated endocytosis; synaptogenesis; immune system process; apoptosis; Rac protein signal transduction; neurite morphogenesis; activation of JNK activity; cell structure disassembly during apoptosis