Mouse anti-Human ABHD5 Monoclonal Antibody | anti-ABHD5 antibody

ABHD5 (1-acylglycerol-3-phosphate O-acyltransferase ABHD5, Abhydrolase Domain-containing Protein 5, Lipid Droplet-binding Protein CGI-58, CGI-58, MGC8731, NCIE2) (Biotin)

Cat. Num. AAA6140425

• Gene Names: ABHD5; CDS; CGI58; IECN2; NCIE2
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ABHD5; Monoclonal Antibody; ABHD5 (1-acylglycerol-3-phosphate O-acyltransferase ABHD5; Abhydrolase Domain-containing Protein 5; Lipid Droplet-binding Protein CGI-58; CGI-58; MGC8731; NCIE2) (Biotin); anti-ABHD5 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 1F3
• Specificity: Recognizes human ABHD5.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-ABHD5 antibody: ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IHC-P: 3ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa1-350 from human ABHD5 (AAH21958) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MAAEEEEVDSADTGERSGWLTGWLPTWCPTSISHLKEAEEKMLKCVPCTYKKEPVRISNGNKIWTLKFSHNISNKTPLVLLHGFGGGLGLWALNFGDLCTNRPVYAFDLLGFGRSSRPRFDSDAEEVENQFVESIEEWRCALGLDKMILLGHNLGGFLAAAYSLKYPSRVNHLILVEPWGFPERPDLADQDRPIPVWIRALGAALTPFNPLAGLRIAGPFGLSLVQRLRPDFKRKYSSMFEDDTVTEYIYHCNVQTPSGETAFKNMTIPYGWAKRPMLQRIGKMHPDIPVSVIFGARSCIDGNSGTSIQSLRPHSYVKTIAILGAGHYVYADQPEEFNQKVKEICDTVD
• Conjugate: Biotin
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (64.5kD).)

Western Blot (WB)

(ABHD5 monoclonal antibody. Western Blot analysis of ABHD5 expression in human liver.)

Western Blot (WB)

(Western Blot analysis of ABHD5 expression in transfected 293T cell line by ABHD5 monoclonal antibody. Lane 1: ABHD5 transfected lysate (39kD). Lane 2: Non-transfected lysate.)

Immunohistochemistry (IHC)

(Immunoperoxidase of monoclonal antibody to ABHD5 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3ug/ml].)

Testing Data

(Detection limit for recombinant GST tagged ABHD5 is ~1ng/ml as a capture antibody.)

Western Blot (WB)

(ABHD5 monoclonal antibody. Western Blot analysis of ABHD5 expression in Jurkat.)

Product Categories/Family for anti-ABHD5 antibody

Antibodies; Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 18314390
• NCBI GeneID: 51099
• Molecular Weight: 39,096 Da
• NCBI Official Full Name: Homo sapiens abhydrolase domain containing 5, mRNA
• NCBI Official Synonym Full Names: abhydrolase domain containing 5
• NCBI Official Symbol: ABHD5
• NCBI Official Synonym Symbols: CDS; CGI58; IECN2; NCIE2
• NCBI Protein Information: 1-acylglycerol-3-phosphate O-acyltransferase ABHD5
• Protein Family: 1-acylglycerol-3-phosphate O-acyltransferase

NCBI Description

The protein encoded by this gene belongs to a large family of proteins defined by an alpha/beta hydrolase fold, and contains three sequence motifs that correspond to a catalytic triad found in the esterase/lipase/thioesterase subfamily. It differs from other members of this subfamily in that its putative catalytic triad contains an asparagine instead of the serine residue. Mutations in this gene have been associated with Chanarin-Dorfman syndrome, a triglyceride storage disease with impaired long-chain fatty acid oxidation. [provided by RefSeq, Jul 2008]