Rabbit TSHZ3 Polyclonal Antibody | anti-TSHZ3 antibody

TSHZ3 Antibody

Cat. Num. AAA150201

• Gene Names: TSHZ3; TSH3; ZNF537
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: TSHZ3 Antibody is affinity chromatography purified via peptide column.
• Synonyms: TSHZ3; Polyclonal Antibody; TSHZ3 Antibody; TSH3; ZNF537; KIAA1474; Teashirt homolog 3; Zinc finger protein 537; teashirt zinc finger homeobox 3; anti-TSHZ3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: TSHZ3 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 1081

• Applicable Applications for anti-TSHZ3 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: TSHZ3 antibody can be used for detection of TSHZ3 by Western blot at 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 2.5 mug/mL.
• Conjugate: Unconjugated
• Immunogen: TSHZ3 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human TSHZ3.
• Buffer: TSHZ3 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: TSHZ3 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of TSHZ3 in mouse brain tissue lysate with TSHZ3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.)

Immunohistochemistry (IHC)

(Immunohistochemistry of TSHZ3 in mouse brain tissue with TSHZ3 antibody at 2.5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of TSHZ3 in mouse brain tissue with TSHZ3 antibody at 20 μg/mL.)

Related Product Information for anti-TSHZ3 antibody

TSHZ3 Antibody: The Teashirt zinc finger homeobox (TSHZ) family comprise a family of evolutionarily conserved transcription factors that, in Drosophila, are active in specific body parts for patterning, but whose function in vertebrates is less clear. In mice, the known three TSHZ proteins are expressed in distinct patterns in the developing and adult brain, suggesting that they play a role in the establishment of regional identity and specification of cell types within the brain. Both TSHZ3 and TSHZ1 have been found to interact with FE65, an adapter protein that binds to the amyloid protein precursor (APP) in neurons. Together with SET, a component of the inhibitor of acetyl transferase, and histone deacetylases, these proteins formed a gene-silencing complex whose target includes caspase-4. Recent experiments have also suggested this family of proteins may be involved in carcinogenesis.

NCBI and Uniprot Product Information

• NCBI GI #: 127138957
• NCBI GeneID: 57616
• NCBI Accession #: NP_065907
• NCBI GenBank Nucleotide #: NM_020856.2
• UniProt Accession #: Q63HK5; Q9H0G6; Q9P254
• Molecular Weight: 118,566 Da
• NCBI Official Full Name: teashirt homolog 3
• NCBI Official Synonym Full Names: teashirt zinc finger homeobox 3
• NCBI Official Symbol: TSHZ3
• NCBI Official Synonym Symbols: TSH3; ZNF537
• NCBI Protein Information: teashirt homolog 3; teashirt 3; zinc finger protein 537; teashirt family zinc finger 3
• UniProt Protein Name: Teashirt homolog 3
• Protein Family: Teashirt
• UniProt Gene Name: TSHZ3
• UniProt Synonym Gene Names: KIAA1474; TSH3; ZNF537
• UniProt Entry Name: TSH3_HUMAN

Uniprot Description

TSHZ3: Transcriptional regulator involved in developmental processes. Function in association with APBB1, SET and HDAC factors as a transcriptional repressor, that inhibits the expression of CASP4. TSHZ3-mediated transcription repression involves the recruitment of histone deacetylases HDAC1 and HDAC2. Associates with chromatin in a region surrounding the CASP4 transcriptional start site(s). Regulates the development of neurons involved in both respiratory rhythm and airflow control. Promotes maintenance of nucleus ambiguus (nA) motoneurons, which govern upper airway function, and establishes a respiratory rhythm generator (RRG) activity compatible with survival at birth. Involved in the differentiation of the proximal uretic smooth muscle cells during developmental processes. Involved in the up- regulation of myocardin, that directs the expression of smooth muscle cells in the proximal ureter. Belongs to the teashirt C2H2-type zinc-finger protein family.

Protein type: C2H2-type zinc finger protein; Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 19q12

Cellular Component: nucleoplasm; growth cone; mitochondrion; nucleus

Molecular Function: protein binding; DNA binding; metal ion binding; chromatin binding

Biological Process: transcription, DNA-dependent; sensory perception of touch; in utero embryonic development; ureteric bud development; positive regulation of smooth muscle cell differentiation; neurological control of breathing; musculoskeletal movement; negative regulation of transcription, DNA-dependent; metanephros development; lung development