Mouse ABCA1 Monoclonal Antibody | anti-ABCA1 antibody

Anti-ABCA1 Antibody

Cat. Num. AAA415413

• Gene Names: ABCA1; TGD; ABC1; CERP; ABC-1; HDLDT1
• Reactivity: human, mouse, other
• Applications: Western Blot, ELISA, Immunoprecipitation, Immunohistochemistry
• Synonyms: ABCA1; Monoclonal Antibody; Anti-ABCA1 Antibody; anti-ABCA1 antibody

• Host: Mouse
• Reactivity: human, mouse, other
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: AB.H10
• Specificity: Recognize specifically ABCA1 from human, mouse, and chicken.
• Form/Format: Liquid; PBS, pH 7.4 with 0.05% sodium azide.
• Concentration: 100ug/100ul (varies by lot)
• Sequence Length: 2261

• Applicable Applications for anti-ABCA1 antibody: Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP), Immunohistochemistry (IHC)
• Application Notes: Starting concentration for WB is 1:1000. This antibody has been tested with direct ELISA, where a 96-well plate was coated with recombinant ABCA1 C-terminal protein. The antibody was then added to the wells and a goat anti-mouse IgG HRP conjugate was used for colour development. For ABCA1 quantification, a pair of anti-ABCA1 antibodies should be used to formulate a Sandwich ELISA and with a presence of an ABCA1 protein Standard. Optimal dilution to be determined by the end user.
• Antibody Type: primary antibody
• Immunogen: Peptides amino acids 1800-2260 (C-Terminal end)
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Western Blot (WB)

Related Product Information for anti-ABCA1 antibody

Mutations in the ABCA1 gene (ATP-binding cassette transporter 1) are associated with Tangier disease (TD). TD is an autosomal recessive disorder results from an absence of plasma HDL, cholesterol ester depositing in the reticulo-endothelial system and disorders in cellular lipid trafficking. It is expressed on the plasma membrane and the Golgi complex, and is regulated by cholesterol flux. Regulation of the cholesterol flux between HDL and macrophages is competitive between ABCA1 and SR-BI.

References

Umeda, T et al. "Regulation of cholesterol efflux by amyloid beta secretion." J. Neurosci. Res. 88:1985-94 (2010). PubMed: 20155813.

NCBI and Uniprot Product Information

• NCBI GI #: 21536376
• NCBI GeneID: 19
• NCBI Accession #: NP_005493.2
• NCBI GenBank Nucleotide #: NM_005502.3
• NCBI Official Full Name: ATP-binding cassette sub-family A member 1
• NCBI Official Synonym Full Names: ATP-binding cassette, sub-family A (ABC1), member 1
• NCBI Official Symbol: ABCA1
• NCBI Official Synonym Symbols: TGD; ABC1; CERP; ABC-1; HDLDT1
• NCBI Protein Information: ATP-binding cassette sub-family A member 1; ATP-binding cassette transporter A1; cholesterol efflux regulatory protein; membrane-bound
• UniProt Protein Name: ATP-binding cassette sub-family A member 1
• Protein Family: ABC transporter A family
• UniProt Gene Name: ABCA1
• UniProt Entry Name: ABCA1_HUMAN

NCBI Description

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. With cholesterol as its substrate, this protein functions as a cholesteral efflux pump in the cellular lipid removal pathway. Mutations in this gene have been associated with Tangier's disease and familial high-density lipoprotein deficiency. [provided by RefSeq, Jul 2008]

Uniprot Description

ABCA1: an ATP-binding cassette (ABC) transporter that regulates cholesterol homeostasis and insulin secretion in beta-cells, and protects against cardiovascular disease. Inactivation of ABCA1 in beta-cells markedly impairs glucose tolerance and insulin secretion in mice. Rosiglitazone, an activator of the peroxisome proliferator-activated receptor-gamma, upregulates ABCA1 in beta-cells. ABCA1 expression in beta-cells is required for the beneficial effects of rosiglitazone on glucose tolerance. Mediates the transport of cholesterol and phospholipids from cells to HDL apolipoproteins, modulating HDL levels and atherogenesis. Induced by bacterial lipopolysaccharides (LPS) and repressed by ZNF202. Phosphorylation on Ser-2054 regulates phospholipid efflux. Unsaturated fatty acids destabilize ABCA1 by activating a PKC-delta pathway that phosphorylates ABCA1 serines. Defects in ABCA1 are a cause of high density lipoprotein deficiencies.

Protein type: Transporter; Vesicle; Membrane protein, multi-pass; Membrane protein, integral; Transporter, ABC family

Chromosomal Location of Human Ortholog: 9q31.1

Cellular Component: Golgi apparatus; perinuclear region of cytoplasm; integral to plasma membrane; endocytic vesicle; plasma membrane; phagocytic vesicle; lipid raft; external side of plasma membrane

Molecular Function: apolipoprotein A-I receptor activity; ATPase activity; cholesterol binding; apolipoprotein binding; ATPase binding; small GTPase binding; apolipoprotein A-I binding; protein binding; phospholipid transporter activity; syntaxin binding; cholesterol transporter activity; phospholipid binding; anion transmembrane transporter activity; ATP binding; receptor binding

Biological Process: response to drug; cholesterol metabolic process; regulation of Cdc42 protein signal transduction; intracellular cholesterol transport; lipoprotein metabolic process; cholesterol efflux; cellular lipid metabolic process; endosome transport; phospholipid translocation; G-protein coupled receptor protein signaling pathway; cholesterol homeostasis; reverse cholesterol transport; platelet dense granule organization and biogenesis; phospholipid efflux; lysosome organization and biogenesis; response to low density lipoprotein stimulus; positive regulation of cAMP biosynthetic process; protein amino acid lipidation; interleukin-1 beta secretion; phagocytosis, engulfment; response to nutrient; phospholipid homeostasis; peptide secretion

Disease: Hypoalphalipoproteinemia, Primary; Tangier Disease; Hypercholesterolemia, Familial