ELISA (EIA)
(Figure 1. ELISA plate pre-coated by 2 ug/ml (100 ul/well) Human 4-1BB Protein, mFc-His Tag can bind Rabbit anti-4-1BB monoclonal antibody (clone: DM67) in a linear range of 1-100 ng/ml.)
Rabbit anti-Human 4-1BB Monoclonal Antibody | anti-4-1BB antibody
Anti-4-1BB antibody (DM67), Rabbit mAb
FC/FACS: 1:100
ELISA (EIA)
(Figure 1. ELISA plate pre-coated by 2 ug/ml (100 ul/well) Human 4-1BB Protein, mFc-His Tag can bind Rabbit anti-4-1BB monoclonal antibody (clone: DM67) in a linear range of 1-100 ng/ml.)
ELISA (EIA)
(Figure 1. ELISA plate pre-coated by 2 ug/ml (100 ul/well) Human 4-1BB Protein, mFc-His Tag can bind Rabbit anti-4-1BB monoclonal antibody (clone: DM67) in a linear range of 1-100 ng/ml.)
Testing Data
(Figure 2. Expi 293 cell line transfected with irrelevant protein (A) and human 4-1BB (B) were surface stained with Rabbit anti-4-1BB monoclonal antibody 1ug/ml (clone: DM67) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.)
Testing Data
(Figure 2. Expi 293 cell line transfected with irrelevant protein (A) and human 4-1BB (B) were surface stained with Rabbit anti-4-1BB monoclonal antibody 1ug/ml (clone: DM67) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.)
Flow Cytometry (FC/FACS)
(Figure 3. Flow cytometry data of serially titrated Rabbit anti-4-1BB monoclonal antibody (clone: DM67) on Expi 293 cell line transfected with human 4-1BB. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)
Flow Cytometry (FC/FACS)
(Figure 3. Flow cytometry data of serially titrated Rabbit anti-4-1BB monoclonal antibody (clone: DM67) on Expi 293 cell line transfected with human 4-1BB. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)
Testing Data
(Figure 4. Affinity ranking of different Rabbit anti-4-1BB mAb clones by titration of different concentration onto Expi 293 cell line transfected with human 4-1BB. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)
Testing Data
(Figure 4. Affinity ranking of different Rabbit anti-4-1BB mAb clones by titration of different concentration onto Expi 293 cell line transfected with human 4-1BB. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contributes to the clonal expansion, survival, and development of T cells. It can also induce proliferation in peripheral monocytes, enhance T cell apoptosis induced by TCR/CD3 triggered activation, and regulate CD28 co-stimulation to promote Th1 cell responses. The expression of this receptor is induced by lymphocyte activation. TRAF adaptor proteins have been shown to bind to this receptor and transduce the signals leading to activation of NF-kappaB. [provided by RefSeq, Jul 2008]
Uniprot Description
TNFRSF9: Receptor for TNFSF9/4-1BBL. Possibly active during T cell activation.
Protein type: Membrane protein, integral; Receptor, misc.
Chromosomal Location of Human Ortholog: 1p36
Cellular Component: extracellular space; integral to plasma membrane; external side of plasma membrane
Molecular Function: cytokine binding; receptor activity
Biological Process: negative regulation of cell proliferation; apoptosis
Research Articles on 4-1BB
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Product Notes
The 4-1BB tnfrsf9 (Catalog #AAA188615) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-4-1BB antibody (DM67), Rabbit mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's 4-1BB can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Flow Cytometry (FC/FACS). ELISA: 1:5000-10,000 FC/FACS: 1:100. Researchers should empirically determine the suitability of the 4-1BB tnfrsf9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "4-1BB, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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