Western Blot (WB)
(Western blot analysis of Casein Kinase Igamma1 using COS7 whole cell lysates)
Rabbit Casein Kinase Igamma1 Polyclonal Antibody | anti-CSNK1G1 antibody
Casein Kinase Igamma1 Antibody
Gene Names
CSNK1G1; CK1gamma1
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
Casein Kinase Igamma1; Polyclonal Antibody; Casein Kinase Igamma1 Antibody; 9130020E21Rik; Casein kinase 1 gamma 1; Casein kinase I isoform gamma 1; Casein kinase I isoform gamma-1; CKI gamma 1; CKI-gamma 1; CSNK1G1; EC 2.7.11.1; KC1G1_HUMAN; anti-CSNK1G1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Monkey
Clonality
Polyclonal
Isotype
IgG
Specificity
Casein Kinase Igamma1 antibody detects endogenous levels of total Casein Kinase Igamma1
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
422
Applicable Applications for anti-CSNK1G1 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:1000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide derived from human Casein Kinase Igamma1
Subcellular Location
Cytoplasm.
Predicted Cross Reactivity
Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Similarity
Pig (83%), Zebrafish (92%), Bovine (92%), Horse (100%), Sheep (92%), Rabbit (100%), Dog (100%), Chicken (83%), Xenopus (83%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of Casein Kinase Igamma1 using COS7 whole cell lysates)
Western Blot (WB)
(Western blot analysis of Casein Kinase Igamma1 using COS7 whole cell lysates)
Immunofluorescene (IF)
(MBS9602076 staining COS7 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescene (IF)
(MBS9602076 staining COS7 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Related Product Information for anti-CSNK1G1 antibody
Function: Serine/threonine-protein kinase. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Regulates fast synaptic transmission mediated by glutamate (By similarity). Phosphorylates CLSPN.
Subunit Structure: Monomer.
Post-translational Modifications: Autophosphorylated.
Similarity: Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
Subunit Structure: Monomer.
Post-translational Modifications: Autophosphorylated.
Similarity: Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 49 kDa
Predicted: 49 kDa
Predicted: 49 kDa
NCBI Official Full Name
casein kinase I isoform gamma-1 isoform 1
NCBI Official Synonym Full Names
casein kinase 1 gamma 1
NCBI Official Symbol
CSNK1G1
NCBI Official Synonym Symbols
CK1gamma1
NCBI Protein Information
casein kinase I isoform gamma-1
UniProt Protein Name
Casein kinase I isoform gamma-1
Protein Family
UniProt Gene Name
CSNK1G1
UniProt Synonym Gene Names
CKI-gamma 1
NCBI Description
This gene encodes a member of the casein kinase I gene family. This family is comprised of serine/threonine kinases that phosphorylate acidic proteins such as caseins. The encoded kinase plays a role in cell cycle checkpoint arrest in response to stalled replication forks by phosphorylating Claspin. A mutation in this gene may be associated with non-syndromic early-onset epilepsy (NSEOE). [provided by RefSeq, Jul 2016]
Uniprot Description
Serine/threonine-protein kinase. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Regulates fast synaptic transmission mediated by glutamate (). Phosphorylates CLSPN.
Research Articles on CSNK1G1
Similar Products
Product Notes
The CSNK1G1 csnk1g1 (Catalog #AAA9602076) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Casein Kinase Igamma1 Antibody reacts with Human, Mouse, Rat, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's Casein Kinase Igamma1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the CSNK1G1 csnk1g1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Casein Kinase Igamma1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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