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Western Blot (WB) (Fig-1: Western blot analysis of 14-3-3 Theta. Anti-14-3-3 Theta antibody (Clone: ABM52H6) was used at 2 ug/ml on mBrain lysate.)

Rat anti-Mouse 14-3-3 Theta Monoclonal Antibody | anti-YWHAQ antibody

Monoclonal Antibody to 14-3-3 Theta (Clone: ABM52H6)

Gene Names
YWHAQ; 1C5; HS1; 14-3-3
Reactivity
Mouse
Applications
Western Blot
Purity
Purified
Protein G Chromatography
Synonyms
14-3-3 Theta; Monoclonal Antibody; Monoclonal Antibody to 14-3-3 Theta (Clone: ABM52H6); 14-3-3 protein T-cell; 14-3-3 protein tau; Protein HS1; 14-3-3 protein theta; anti-YWHAQ antibody
Ordering
For Research Use Only!
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG2a kappa
Clone Number
ABM52H6
Purity/Purification
Purified
Protein G Chromatography
Form/Format
25 ug in 50 ul/100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Sequence Length
245
Applicable Applications for anti-YWHAQ antibody
Western Blot (WB)
Application Notes
WB: 2-4 ug/ml
Immunogen Information
Full length recombinant protein of 14-3-3 Theta was used as the immunogen for this antibody.
Antigen
14-3-3 Theta
Preparation and Storage
Store the antibody at 4 degree C, stable for 6 months.
For long-term storage, store at -20 degree C.
Avoid repeated freeze and thaw cycles.

Western Blot (WB)

(Fig-1: Western blot analysis of 14-3-3 Theta. Anti-14-3-3 Theta antibody (Clone: ABM52H6) was used at 2 ug/ml on mBrain lysate.)

Western Blot (WB) (Fig-1: Western blot analysis of 14-3-3 Theta. Anti-14-3-3 Theta antibody (Clone: ABM52H6) was used at 2 ug/ml on mBrain lysate.)
Related Product Information for anti-YWHAQ antibody
14-3-3 Theta, a member of 14-3-3 family, is an adapter protein that plays an important role in a wide range of vital regulatory processes, including signal transduction, apoptosis, cell cycle progression and DNA replication. Humans have seven 14-3-3 isoforms (beta, gamma, epsilon, eta, sigma, theta and zeta) that serve as scaffolds to promote interactions of regulatory phospho-proteins involved in many vital cellular processes. 14-3-3 Theta binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif, resulting in the modulation of the activity of the binding partner except PDPK1, whose kinase activity is negatively regulated. Previous studies have shown that disturbances in native 14-3-3 levels can contribute significantly to the development of various cancers. In lung cancer biopsies, 14-3-3 Theta is found to be present in abundance.
Product Categories/Family for anti-YWHAQ antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
27,764 Da
NCBI Official Full Name
14-3-3 protein theta
NCBI Official Synonym Full Names
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta
NCBI Official Symbol
YWHAQ
NCBI Official Synonym Symbols
1C5; HS1; 14-3-3
NCBI Protein Information
14-3-3 protein theta
UniProt Protein Name
14-3-3 protein theta
Protein Family
UniProt Gene Name
YWHAQ

NCBI Description

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease. [provided by RefSeq, Jul 2008]

Uniprot Description

14-3-3 theta: a protein of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. A multifunctional regulator of the cell signaling processes. Associates with c-BCR and BCR-Abl.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 2p25.1

Cellular Component: cytoplasm; cytoplasmic vesicle membrane; cytosol; focal adhesion; membrane; protein complex

Molecular Function: protein binding; protein N-terminus binding

Biological Process: negative regulation of transcription, DNA-dependent; substantia nigra development

Research Articles on YWHAQ

Similar Products

Product Notes

The YWHAQ ywhaq (Catalog #AAA668272) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to 14-3-3 Theta (Clone: ABM52H6) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's 14-3-3 Theta can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 2-4 ug/ml. Researchers should empirically determine the suitability of the YWHAQ ywhaq for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "14-3-3 Theta, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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