Monkey TSLP ELISA Kit | TSLP elisa kit

Monkey TSLP (Thymic Stromal Lymphopoietin) ELISA Kit

Cat. Num. AAA2512153

• Reactivity: Monkey
• Synonyms: TSLP; Monkey TSLP (Thymic Stromal Lymphopoietin) ELISA Kit; TSLP elisa kit

• Reactivity: Monkey
• Specificity: This kit recognizes Monkey TSLP in samples. No significant cross-reactivity or interference between Monkey TSLP and analogues was observed.
• Sequence Length: 159

• Samples: Serum, plasma and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 31.25-2000pg/mL
• Sensitivity: 18.75pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Monkey TSLP were tested 20 times on one plate, respectively.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Monkey TSLP were tested on 3 different plates, 20 replicates in each plate.
• Preparation and Storage: Store at 4 degree C.

Related Product Information for TSLP elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Monkey TSLP concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey TSLP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Monkey TSLP and Avidin- Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Monkey TSLP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Monkey TSLP. You can calculate the concentration of Monkey TSLP in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 14719428
• NCBI GeneID: 85480
• NCBI Accession #: NP_149024.1
• NCBI GenBank Nucleotide #: NM_033035.4
• UniProt Accession #: Q969D9; Q8IW99; Q96AU7; G3XAM8
• Molecular Weight: 7,426 Da
• NCBI Official Full Name: thymic stromal lymphopoietin isoform 1
• NCBI Official Synonym Full Names: thymic stromal lymphopoietin
• NCBI Official Symbol: TSLP
• NCBI Protein Information: thymic stromal lymphopoietin
• UniProt Protein Name: Thymic stromal lymphopoietin
• Protein Family: Thymic stromal lymphopoietin
• UniProt Gene Name: TSLP
• UniProt Entry Name: TSLP_HUMAN

NCBI Description

This gene encodes a hemopoietic cytokine proposed to signal through a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor and the IL-7R alpha chain. It mainly impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes and enhances the maturation of CD11c(+) dendritic cells. The protein promotes T helper type 2 (TH2) cell responses that are associated with immunity in various inflammatory diseases, including asthma, allergic inflammation and chronic obstructive pulmonary disease. The protein is therefore considered a potential therapeutic target for the treatment of such diseases. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2012]

Uniprot Description

TSLP: Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c(+) dendritic cells. Can induce allergic inflammation by directly activating mast cells.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5q22.1

Cellular Component: extracellular space

Molecular Function: cytokine activity