Monkey RBP4 ELISA Kit | RBP4 elisa kit

Monkey RBP4 (Retinol Binding Protein 4, Plasma) ELISA Kit

Cat. Num. AAA2510499

• Gene Names: RBP4; RDCCAS
• Reactivity: Monkey
• Synonyms: RBP4; Monkey RBP4 (Retinol Binding Protein 4; Plasma) ELISA Kit; RBP4 elisa kit

• Reactivity: Monkey
• Specificity: This kit recognizes Monkey RBP4 in samples. No significant cross-reactivity or interference between Monkey RBP4 and analogues was observed.
• Sequence Length: 201

• Samples: Serum, plasma and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 1.56-100ng/mL
• Sensitivity: 0.94ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Monkey RBP4 were tested 20 times on one plate, respectively.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Monkey RBP4 were tested on 3 different plates, 20 replicates in each plate.
• Preparation and Storage: Store at 4 degree C.

Related Product Information for RBP4 elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Monkey RBP4 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey RBP4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Monkey RBP4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Monkey RBP4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Monkey RBP4. You can calculate the concentration of Monkey RBP4 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 55743122
• NCBI GeneID: 5950
• NCBI Accession #: NP_006735.2
• NCBI GenBank Nucleotide #: NM_006744.3
• UniProt Accession #: P02753; O43478; O43479; Q5VY24; Q8WWA3; Q9P178; D3DR38
• Molecular Weight: 23,010 Da
• NCBI Official Full Name: retinol-binding protein 4
• NCBI Official Synonym Full Names: retinol binding protein 4, plasma
• NCBI Official Symbol: RBP4
• NCBI Official Synonym Symbols: RDCCAS
• NCBI Protein Information: retinol-binding protein 4; PRBP; plasma retinol-binding protein; retinol-binding protein 4, interstitial
• UniProt Protein Name: Retinol-binding protein 4
• Protein Family: Retinol-binding protein
• UniProt Gene Name: RBP4
• UniProt Synonym Gene Names: PRBP; RBP
• UniProt Entry Name: RET4_HUMAN

NCBI Description

This protein belongs to the lipocalin family and is the specific carrier for retinol (vitamin A alcohol) in the blood. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein posttranslationally and results in defective delivery and supply to the epidermal cells. [provided by RefSeq, Jul 2008]

Uniprot Description

RBP4: Delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, this prevents its loss by filtration through the kidney glomeruli. Defects in RBP4 are a cause of retinol-binding protein deficiency (RBP deficiency). This condition causes night vision problems. It produces a typical 'fundus xerophthalmicus', featuring a progressed atrophy of the retinal pigment epithelium. Belongs to the calycin superfamily. Lipocalin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 10q23.33

Cellular Component: extracellular space; protein complex; extracellular region; cytosol

Molecular Function: protein binding; protein heterodimerization activity; retinal binding; retinol binding

Biological Process: phototransduction, visible light; retinal metabolic process; embryonic skeletal development; heart development; maintenance of gastrointestinal epithelium; uterus development; glucose homeostasis; response to insulin stimulus; embryonic organ morphogenesis; negative regulation of cardiac muscle cell proliferation; retinol metabolic process; detection of light stimulus involved in visual perception; retinoid metabolic process; female genitalia morphogenesis; cardiac muscle development; response to retinoic acid; urinary bladder development; male gonad development; positive regulation of insulin secretion; vagina development; positive regulation of immunoglobulin secretion; gluconeogenesis; response to ethanol; eye development; embryonic retina morphogenesis in camera-type eye; spermatogenesis; lung development

Disease: Retinal Dystrophy, Iris Coloboma, And Comedogenic Acne Syndrome; Microphthalmia, Isolated, With Coloboma 10