Human Cytokine receptor common subunit gamma ELISA Kit | IL2RG elisa kit

Human Cytokine receptor common subunit gamma ELISA Kit

Cat. Num. AAA762946

• Gene Names: IL2RG; P64; CIDX; IMD4; CD132; SCIDX; IL-2RG; SCIDX1
• Reactivity: Human
• Synonyms: Cytokine receptor common subunit gamma; Human Cytokine receptor common subunit gamma ELISA Kit; IL2RG/p64/Interleukin-2 receptor subunit gamma (IL-2 receptor subunit gamma/IL-2R subunit gamma/IL-2RG)/gammaC/CD132/IL-2 R gamma/IL2RG/CD132/CD132 antigen/CIDX/combined immunodeficiency; X-linked/common cytokine receptor gamma chain/common gamma-chain/cytokine receptor common subunit gamma/gamma (c)/IL-2 receptor subunit gamma/IL-2R subunit gamma/IL-2RG/IMD4/interleukin 2 receptor; gamma/Interleukin-2 receptor subunit gamma/P64/p64/SCIDX/SCIDX1/severe combined immunodeficiency; IL2RG elisa kit

• Reactivity: Human
• Sequence Length: 369

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for IL2RG elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology anti-Human IL2RG antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human IL2RG antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human IL2RG amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human IL2RG can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4557882
• NCBI GeneID: 3561
• NCBI Accession #: NP_000197.1
• NCBI GenBank Nucleotide #: NM_000206.2
• Molecular Weight: 20,088 Da
• NCBI Official Full Name: cytokine receptor common subunit gamma
• NCBI Official Synonym Full Names: interleukin 2 receptor subunit gamma
• NCBI Official Symbol: IL2RG
• NCBI Official Synonym Symbols: P64; CIDX; IMD4; CD132; SCIDX; IL-2RG; SCIDX1
• NCBI Protein Information: cytokine receptor common subunit gamma
• UniProt Protein Name: Cytokine receptor common subunit gamma
• Protein Family: Cytokine receptor
• UniProt Gene Name: IL2RG
• UniProt Synonym Gene Names: IL-2 receptor subunit gamma; IL-2R subunit gamma; IL-2RG
• UniProt Entry Name: IL2RG_HUMAN

NCBI Description

The protein encoded by this gene is an important signaling component of many interleukin receptors, including those of interleukin -2, -4, -7 and -21, and is thus referred to as the common gamma chain. Mutations in this gene cause X-linked severe combined immunodeficiency (XSCID), as well as X-linked combined immunodeficiency (XCID), a less severe immunodeficiency disorder. [provided by RefSeq, Mar 2010]

Uniprot Description

IL2RG: Common subunit for the receptors for a variety of interleukins. Defects in IL2RG are the cause of severe combined immunodeficiency X-linked T-cell-negative/B-cell-positive/NK-cell- negative (XSCID); also known as agammaglobulinemia Swiss type. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell- mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development. Defects in IL2RG are the cause of X-linked combined immunodeficiency (XCID). XCID is a less severe form of X-linked immunodeficiency with a less severe degree of deficiency in cellular and humoral immunity than that seen in XSCID. Belongs to the type I cytokine receptor family. Type 5 subfamily.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: Xq13.1

Cellular Component: external side of plasma membrane; integral to plasma membrane; intracellular; membrane; plasma membrane

Molecular Function: interleukin-2 binding; interleukin-2 receptor activity; interleukin-4 receptor activity; interleukin-7 binding; interleukin-7 receptor activity; protein binding

Biological Process: activation of MAPKK activity; axon guidance; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; immune response; innate immune response; insulin receptor signaling pathway; MAPKKK cascade; nerve growth factor receptor signaling pathway; Ras protein signal transduction; signal transduction; small GTPase mediated signal transduction; vascular endothelial growth factor receptor signaling pathway; viral reproduction

Disease: Combined Immunodeficiency, X-linked; Severe Combined Immunodeficiency, X-linked