Monkey CENPF ELISA Kit | CENPF elisa kit
Monkey CENPF (Centromere Protein F) ELISA Kit
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CENPF. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CENPF and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CENPF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of CENPF. You can calculate the concentration of CENPF in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein that associates with the centromere-kinetochore complex. The protein is a component of the nuclear matrix during the G2 phase of interphase. In late G2 the protein associates with the kinetochore and maintains this association through early anaphase. It localizes to the spindle midzone and the intracellular bridge in late anaphase and telophase, respectively, and is thought to be subsequently degraded. The localization of this protein suggests that it may play a role in chromosome segregation during mitotis. It is thought to form either a homodimer or heterodimer. Autoantibodies against this protein have been found in patients with cancer or graft versus host disease. [provided by RefSeq, Jul 2008]
Uniprot Description
CENPF: a nuclear matrix protein that assembles onto kinetochores at late G2. Is hyperphosphorylated during mitosis and rapidly degraded after mitosis. Involved in chromosome segregation during mitosis. Interacts with retinoblastoma protein (RB), CENP-E and BUBR1. Two alternatively spliced human isoforms have been described.
Protein type: Nuclear envelope; Transcription, coactivator/corepressor; Cell cycle regulation
Chromosomal Location of Human Ortholog: 1q41
Cellular Component: spindle pole; nuclear matrix; pronucleus; nuclear envelope; cytosol; nucleoplasm; kinetochore; perinuclear region of cytoplasm; cytoplasm; outer kinetochore of condensed chromosome; spindle; midbody; nucleus; chromatin; chromosome, pericentric region
Molecular Function: protein C-terminus binding; dynein binding; protein binding; protein homodimerization activity; chromatin binding; transcription factor binding
Biological Process: response to drug; mitosis; muscle development; kinetochore assembly; regulation of cell cycle; chromosome segregation; cell proliferation; protein transport; cell division; regulation of G2/M transition of mitotic cell cycle; mitotic cell cycle spindle assembly checkpoint; metaphase plate congression; mitotic cell cycle; cell differentiation; G2/M transition of mitotic cell cycle; negative regulation of transcription, DNA-dependent; regulation of striated muscle development
Disease: Ciliary Dyskinesia, Primary, 31
Research Articles on CENPF
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Product Notes
The Monkey CENPF cenpf (Catalog #AAA2512010) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2512010 ELISA Kit recognizes Monkey CENPF. It is sometimes possible for the material contained within the vial of "CENPF, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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