Typical Testing Data/Standard Curve
Monkey Butyrophilin-like protein 2 (BTNL2) ELISA Kit | BTNL2 elisa kit
Monkey Butyrophilin-like protein 2 (BTNL2) ELISA Kit
Gene Names
BTNL2; SS2; BTN7; BTL-II; HSBLMHC1
Reactivity
Monkey
Synonyms
Butyrophilin-like protein 2 (BTNL2); Monkey Butyrophilin-like protein 2 (BTNL2) ELISA Kit; BTNL2 elisa kit
Reactivity
Monkey
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
1.0 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve
Typical Testing Data/Standard Curve
Related Product Information for BTNL2 elisa kit
Intended Uses: This BTNL2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey BTNL2. This ELISA kit for research use only!
Principle of the Assay||BTNL2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-BTNL2 antibody and an BTNL2-HRP conjugate. The assay sample and buffer are incubated together with BTNL2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BTNL2 concentration since BTNL2 from samples and BTNL2-HRP conjugate compete for the anti-BTNL2 antibody binding site. Since the number of sites is limited, as more sites are occupied by BTNL2 from the sample, fewer sites are left to bind BTNL2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BTNL2 concentration in each sample is interpolated from this standard curve.
Principle of the Assay||BTNL2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-BTNL2 antibody and an BTNL2-HRP conjugate. The assay sample and buffer are incubated together with BTNL2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BTNL2 concentration since BTNL2 from samples and BTNL2-HRP conjugate compete for the anti-BTNL2 antibody binding site. Since the number of sites is limited, as more sites are occupied by BTNL2 from the sample, fewer sites are left to bind BTNL2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BTNL2 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for BTNL2 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28,660 Da
NCBI Official Full Name
butyrophilin-like protein 2
NCBI Official Synonym Full Names
butyrophilin-like 2 (MHC class II associated)
NCBI Official Symbol
BTNL2
NCBI Official Synonym Symbols
SS2; BTN7; BTL-II; HSBLMHC1
NCBI Protein Information
butyrophilin-like protein 2
UniProt Protein Name
Butyrophilin-like protein 2
Protein Family
UniProt Gene Name
BTNL2
UniProt Synonym Gene Names
BTL-II
Uniprot Description
BTNL2: Negative regulator of T-cell proliferation. Genetic variation in BTNL2 is a cause of susceptibility to sarcoidosis type 2 (SS2). Sarcoidosis is an idiopathic, systemic, inflammatory disease characterized by the formation of immune granulomas in involved organs. Granulomas predominantly invade the lungs and the lymphatic system, but also skin, liver, spleen, eyes and other organs may be involved. A nucleotide transition affecting a splice donor site results in the use of an alternative splice site and the production of isoform 3. Individuals expressing isoform 3 have a higher risk for sarcoidosis. Belongs to the immunoglobulin superfamily. BTN/MOG family. 6 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 6p21.3
Cellular Component: integral to membrane
Disease: Sarcoidosis, Susceptibility To, 2
Research Articles on BTNL2
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Product Notes
The Monkey BTNL2 btnl2 (Catalog #AAA7217239) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7217239 ELISA Kit recognizes Monkey BTNL2. It is sometimes possible for the material contained within the vial of "Butyrophilin-like protein 2 (BTNL2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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