Typical Testing Data/Standard Curve (for reference only)
Human Carbohydrate Sulfotransferase 15 (CHST15) ELISA Kit | CHST15 elisa kit
Human Carbohydrate Sulfotransferase 15 (CHST15) ELISA Kit
Gene Names
Chst15; BRAG; MAd5; mKIAA0598; GalNAcS-6ST; GalNAc4S-6ST; 4631426J05Rik
Reactivity
Human
Synonyms
Carbohydrate Sulfotransferase 15 (CHST15); Human Carbohydrate Sulfotransferase 15 (CHST15) ELISA Kit; CHST15 elisa kit
Reactivity
Human
Sequence Length
561
Samples
Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
0.1ng/ml - 40ng/ml.
Sensitivity
0.043ng/ml
Intra-Assay
CV<8%
Inter-Assay
CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CHST15 elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of human Carbonydrate Sulfortransferase 15 (also known as CHST15) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human CHST15 antibody. CHST15 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human CHST15 Antibody is added and binds to CHST15 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CHST15 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human CHST15. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human CHST15 antibody. CHST15 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human CHST15 Antibody is added and binds to CHST15 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CHST15 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human CHST15. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
64,986 Da
NCBI Official Full Name
Carbohydrate sulfotransferase 15
NCBI Official Synonym Full Names
carbohydrate (N-acetylgalactosamine 4-sulfate 6-O) sulfotransferase 15
NCBI Official Symbol
Chst15
NCBI Official Synonym Symbols
BRAG; MAd5; mKIAA0598; GalNAcS-6ST; GalNAc4S-6ST; 4631426J05Rik
NCBI Protein Information
carbohydrate sulfotransferase 15
UniProt Protein Name
Carbohydrate sulfotransferase 15
Protein Family
UniProt Gene Name
Chst15
UniProt Synonym Gene Names
Brag; Galnac4s6st; Kiaa0598; GalNAc4S-6ST
Uniprot Description
Sulfotransferase that transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to the C-6 hydroxyl group of the GalNAc 4-sulfate residue of chondroitin sulfate A and forms chondroitin sulfate E containing GlcA-GalNAc(4,6-SO4) repeating units. It also transfers sulfate to a unique non-reducing terminal sequence, GalNAc(4SO4)-GlcA(2SO4)-GalNAc(6SO4), to yield a highly sulfated structure similar to the structure found in thrombomodulin chondroitin sulfate. May also act as a B-cell receptor involved in BCR ligation-mediated early activation that mediate regulatory signals key to B-cell development and/or regulation of B-cell-specific RAG expression; however such results are unclear in vivo ().
Research Articles on CHST15
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Product Notes
The Human CHST15 chst15 (Catalog #AAA160625) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA160625 ELISA Kit recognizes Human CHST15. It is sometimes possible for the material contained within the vial of "Carbohydrate Sulfotransferase 15 (CHST15), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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