MMP19 recombinant protein

MMP19 Protein, Human, Recombinant

Cat. Num. AAA8121575

• Gene Names: MMP19; MMP18; RASI-1
• Purity: >85% as determined by SDS-PAGE
• Synonyms: MMP19; MMP19 Protein; Human; Recombinant; Human RASI-1/MMP19 Protein; MMP18 Protein; RASI-1 Protein; matrix metallopeptidase 19; MMP19 recombinant protein

• Host: E Coli
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 100mM NaCl, 0.5M Arg, 5mM CaCl2, 0.03% Brij35, 50uM ZnCl2, 1/0.1mM GSH/GSSG, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffe
• Sequence: Leu101-Gly256

• Species: Human
• Predicted N Terminal: Met
• Tag: Native
• Protein Construction: A DNA sequence encoding the catalytic domain of human MMP19 (Q99542-1)(Leu101-Gly256) was expressed, with a N-terminal Met.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for MMP19 recombinant protein

Background: MMP19, also known as RASI-1, is a member of the peptidase M1A family. It contains 4 hemopexin-like domains and is expressed in the mammary gland, placenta, lung, pancreas, ovary, small intestine, spleen, thymus, prostate, testis colon, heart, and blood vessel walls. It is a matrix metalloproteinase (MMP). Proteins of the MMP family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. MMP19 may play a role in pathological processes participating in rheumatoid arthritis (RA)-associated joint tissue destruction. Autoantigen anti-MMP19 is frequent in RA patients.

References

Cossins J., et al.,(1996), Identification of MMP-18, a putative novel human matrix metalloproteinase. Biochem. Biophys. Res. Commun. 228:494-498.Pendas A.M., et al., (1997), Identification and characterization of a novel human matrix metalloproteinase with unique structural characteristics, chromosomal location and tissue distribution.J. Biol. Chem. 272:4281-4286.Kolb C., et al.,(1997), The matrix metalloproteinase RASI-1 is expressed in synovial blood vessels of a rheumatoid arthritis patient.Immunol. Lett. 57:83-88.

NCBI and Uniprot Product Information

• NCBI GI #: 442535505
• NCBI GeneID: 4327
• NCBI Accession #: NP_001259030.1
• NCBI GenBank Nucleotide #: NM_001272101.1
• UniProt Accession #: Q99542; O15278; O95606; Q99580
• Molecular Weight: 57,357 Da
• NCBI Official Full Name: matrix metalloproteinase-19 isoform 3 preproprotein
• NCBI Official Synonym Full Names: matrix metallopeptidase 19
• NCBI Official Symbol: MMP19
• NCBI Official Synonym Symbols: MMP18; RASI-1
• NCBI Protein Information: matrix metalloproteinase-19; matrix metalloproteinase-18; matrix metalloproteinase RASI; matrix metalloproteinase-beta19
• UniProt Protein Name: Matrix metalloproteinase-19
• Protein Family: Matrix metalloproteinase
• UniProt Gene Name: MMP19
• UniProt Synonym Gene Names: MMP18; RASI; MMP-19; MMP-18
• UniProt Entry Name: MMP19_HUMAN

NCBI Description

This gene encodes a member of a family of proteins that are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded protein is secreted as an inactive proprotein, which is activated upon cleavage by extracellular proteases. Alternative splicing results in multiple transcript variants for this gene. [provided by RefSeq, Jan 2013]

Uniprot Description

MMP19: Endopeptidase that degrades various components of the extracellular matrix, such as aggrecan and cartilage oligomeric matrix protein (comp), during development, haemostasis and pathological conditions (arthritic disease). May also play a role in neovascularization or angiogenesis. Hydrolyzes collagen type IV, laminin, nidogen, nascin-C isoform, fibronectin, and type I gelatin. May play a role in pathological processes participating in rheumatoid arthritis (RA)-associated joint tissue destruction. Autoantigen anti-MMP19 are frequent in RA patients. Belongs to the peptidase M10A family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; EC 3.4.24.-; Secreted, signal peptide; Protease

Chromosomal Location of Human Ortholog: 12q14

Cellular Component: proteinaceous extracellular matrix; extracellular region

Molecular Function: zinc ion binding; metalloendopeptidase activity; calcium ion binding

Biological Process: collagen catabolic process; extracellular matrix disassembly; extracellular matrix organization and biogenesis; response to cAMP; luteolysis; ovarian follicle development; response to hormone stimulus; angiogenesis; ovulation from ovarian follicle; cell differentiation; proteolysis