Human Matrix Metalloproteinase 11 ELISA Kit | MMP11 elisa kit

Human Matrix Metalloproteinase 11 ELISA Kit

Cat. Num. AAA762391

• Gene Names: MMP11; ST3; SL-3; STMY3
• Reactivity: Human
• Synonyms: Matrix Metalloproteinase 11; Human Matrix Metalloproteinase 11 ELISA Kit; MMP-11 (Matrix Metalloproteinase 11)/MMP11/SL-3/ST3/STMY3/Stromelysin-3/stromelysin 3/stromelysin III; MMP11 elisa kit

• Reactivity: Human
• Sequence Length: 67

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for MMP11 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human MMP11 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human MMP11 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human MMP11 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human MMP11 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 90287776
• NCBI GeneID: 4320
• Molecular Weight: 54,590 Da
• NCBI Official Full Name: matrix metalloproteinase 11, partial
• NCBI Official Synonym Full Names: matrix metallopeptidase 11
• NCBI Official Symbol: MMP11
• NCBI Official Synonym Symbols: ST3; SL-3; STMY3
• NCBI Protein Information: stromelysin-3
• UniProt Protein Name: Stromelysin-3
• Protein Family: Stromelysin
• UniProt Gene Name: MMP11
• UniProt Synonym Gene Names: STMY3; SL-3; ST3; MMP-11
• UniProt Entry Name: MMP11_HUMAN

NCBI Description

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the enzyme encoded by this gene is activated intracellularly by furin within the constitutive secretory pathway. Also in contrast to other MMP's, this enzyme cleaves alpha 1-proteinase inhibitor but weakly degrades structural proteins of the extracellular matrix. [provided by RefSeq, Jul 2008]

Uniprot Description

MMP11: May play an important role in the progression of epithelial malignancies. Belongs to the peptidase M10A family.

Protein type: EC 3.4.24.-; Extracellular matrix; Secreted; Protease; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 22q11.23

Cellular Component: extracellular region; Golgi lumen; proteinaceous extracellular matrix

Molecular Function: calcium ion binding; metalloendopeptidase activity; zinc ion binding

Biological Process: collagen catabolic process; collagen fibril organization; extracellular matrix disassembly; extracellular matrix organization and biogenesis; multicellular organismal development; negative regulation of fat cell differentiation; proteolysis