GALNT1 active protein

Recombinant GALNT1

Cat. Num. AAA201115

• Gene Names: GALNT1; GALNAC-T1
• Applications: SDS-Page
• Purity: >90% by SDS-PAGE
• Synonyms: GALNT1; Recombinant GALNT1; Polypeptide N-acetylgalactosaminyltransferase 1; GALNAC-T1; GALNT1 active protein

• Host: Baculovirus
• Purity/Purification: >90% by SDS-PAGE
• Form/Format: Liquid; In 20mM Tris-HCl Buffer (pH 8.0) Containing 0.1M NaCl, 10% Glycerol.
• Concentration: 1mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence Positions: 41-559aa
• Sequence: ADPGLPAGDV LEPVQKPHEG PGEMGKPVVI PKEDQEKMKE MFKINQFNLM ASEMIALNRS LPDVRLEGCK TKVYPDNLPT TSVVIVFHNE AWSTLLRTVH SVINRSPRHM IEEIVLVDDA SERDFLKRPL ESYVKKLKVP VHVIRMEQRS GLIRARLKGA AVSKGQVITF LDAHCECTVG WLEPLLARIK HDRRTVVCPI IDVISDDTFE YMAGSDMTYG GFNWKLNFRW YPVPQREMDR RKGDRTLPVR TPTMAGGLFS IDRDYFQEIG TYDAGMDIWG GENLEISFRI WQCGGTLEIV TCSHVGHVFR KATPYTFPGG TGQIINKNNR RLAEVWMDEF KNFFYIISPG VTKVDYGDIS SRVGLRHKLQ CKPFSWYLEN IYPDSQIPRH YFSLGEIRNV ETNQCLDNMA RKENEKVGIF NCHGMGGNQV FSYTANKEIR TDDLCLDVSK LNGPVTMLKC HHLKGNQLWE YDPVKLTLQH VNSNQCLDKA TEEDSQVPSI RDCNGSRSQQ WLLRNVTLPE IFHHHHHH
• Sequence Length: 559

• Applicable Applications for GALNT1 active protein: Enzyme Activity, SDS-PAGE
• Species: Human
• Endotoxin: <1.0 EU per 1ug of protein (determined by LAL method)
• Tag: His-Tag
• Predicted N-Terminal: Ala
• Biological Activity: Specific activity is > 300 pmol/min/ug, and is defined as the amount of enzyme that transfer 1.0 pmole of GalNAc from UDP-GalNAc to peptide EA2 per minute per minute at pH 8.0 at 37C.
• Preparation and Storage: Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain )

Related Product Information for GALNT1 active protein

GALNT1, also known as polypeptide N-acetylgalactosaminyltransferase 1, is a member of the GalNAc-T family of enzymes. It utilizes UDP-GalNAc as the nucleotide donor substrate to modify serine or threonine residues of proteins trafficking through the secretory pathway. Structurally, GalNAc-Ts consist of an N-terminal catalytic domain tethered by a short linker to a C-terminal ricin-like lectin domain containing three potential carbohydrate-binding sites. Also, this protein is involved in the glycosylation of proteins essential for bone formation such as osteopontin and bone sialoprotein. Recombinant human GALNT1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for GALNT1 active protein

Signal Transduction; Metabolism

References

Gerken, T.A. et al. (2011) J. Biol. Chem. 286:14493-14507. Partt, M.R. et al. (2004) Chem. Biol. 11:1009-1016.

NCBI and Uniprot Product Information

• NCBI GI #: 13124891
• NCBI GeneID: 2589
• NCBI Accession #: NP_065207.2
• NCBI GenBank Nucleotide #: NP_065207.2
• UniProt Accession #: Q10472
• Molecular Weight: 60.4kDa (528aa); 50-70kDa (SDS-PAGE under reducing conditions)
• NCBI Official Full Name: polypeptide N-acetylgalactosaminyltransferase 1
• NCBI Official Synonym Full Names: polypeptide N-acetylgalactosaminyltransferase 1
• NCBI Official Symbol: GALNT1
• NCBI Official Synonym Symbols: GALNAC-T1
• NCBI Protein Information: polypeptide N-acetylgalactosaminyltransferase 1
• UniProt Protein Name: Polypeptide N-acetylgalactosaminyltransferase 1
• Protein Family: Polypeptide N-acetylgalactosaminyltransferase
• UniProt Gene Name: GALNT1
• UniProt Synonym Gene Names: GalNAc-T1; pp-GaNTase 1

NCBI Description

This gene encodes a member of the UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (GalNAc-T) family of enzymes. GalNAc-Ts initiate mucin-type O-linked glycosylation in the Golgi apparatus by catalyzing the transfer of GalNAc to serine and threonine residues on target proteins. They are characterized by an N-terminal transmembrane domain, a stem region, a lumenal catalytic domain containing a GT1 motif and Gal/GalNAc transferase motif, and a C-terminal ricin/lectin-like domain. GalNAc-Ts have different, but overlapping, substrate specificities and patterns of expression. Transcript variants derived from this gene that utilize alternative polyA signals have been described in the literature. [provided by RefSeq, Jul 2008]

Uniprot Description

Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Has a broad spectrum of substrates for peptides such as EA2, Muc5AC, Muc1a, Muc1b and Muc7.