Human Ki67 ELISA Kit | MKI67 elisa kit

Human Ki67 ELISA Kit

Cat. Num. AAA8291369

• Gene Names: MKI67; KIA; MIB-; MIB-1; PPP1R105
• Reactivity: Human
• Synonyms: Ki67; Human Ki67 ELISA Kit; Antigen KI-67; MKI67 elisa kit

• Reactivity: Human
• Specificity: The Human Ki67 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Ki67 proteins within the range of 15.6 pg/ml - 1000 pg/ml.
• Sequence Length: 2,896

• Samples: Cell culture supernates, serum, plasma, cell lysates, tissue homogenates
• Sensitivity: < 8 pg/ml
• Preparation and Storage: Store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for MKI67 elisa kit

Principle of the Assay: The MyBioSource' Human Ki67 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Ki67 in Cell Culture Supernates, Serum, Plasma, Cell Lysates, Tissue Homogenates. This assay employs an antibody specific for Human Ki67 coated on a 96-well plate. Standards and samples are pipetted into the wells and Ki67 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Ki67 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Ki67 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background/Introduction: Ki-67 (also MKI67and TSG126) is a 350-370 kDa nuclear protein that belongs to a molecular group comprised of mitotic chromosome-associated proteins. Ki-67 was originally recognized as an antigen associated with the monoclonal Ki-67 antibody raised against Hodgkin's lymphoma nuclear material. Ki-67 is contextually expressed, being potentially found in all cells that are not in the Go phase of the cell cycle. Thus, KI-67 qualifies as a cell proliferation marker. Functionally, Ki-67 is known to interact with 160 kDa Hklp2, a protein that promotes centrosome separation and spindle bipolarity. It also directly interacts with NIFK, and apparently binds to UBF, thus playing a role in rRNA synthesis.

NCBI and Uniprot Product Information

• NCBI GI #: 225543215
• NCBI GeneID: 4288
• NCBI Accession #: NP_001139438.1
• NCBI GenBank Nucleotide #: NM_001145966.1
• UniProt Accession #: P46013; Q5VWH2
• Molecular Weight: 319,444 Da
• NCBI Official Full Name: proliferation marker protein Ki-67 isoform 2
• NCBI Official Synonym Full Names: marker of proliferation Ki-67
• NCBI Official Symbol: MKI67
• NCBI Official Synonym Symbols: KIA; MIB-; MIB-1; PPP1R105
• NCBI Protein Information: proliferation marker protein Ki-67; antigen KI-67
• UniProt Protein Name: Proliferation marker protein Ki-67
• Protein Family: Proliferation marker protein
• UniProt Gene Name: MKI67
• UniProt Synonym Gene Names: Antigen KI-67Curated; Antigen Ki67Curated

NCBI Description

This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009]

Uniprot Description

Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable).