Typical Testing Data/Standard Curve (for reference only)
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Guinea Pig Macrophage MigRation Inhibitory Factor ELISA Kit | MIF elisa kit
Guinea pig Macrophage MigRation Inhibitory Factor ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
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Typical Testing Data/Standard Curve (for reference only)
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Principle of the Assay: MIF ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-MIF antibody and an MIF-HRP conjugate. The assay sample and buffer are incubated together with MIF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MIF concentration since MIF from samples and MIF-HRP conjugate compete for the anti-MIF antibody binding site. Since the number of sites is limited, as more sites are occupied by MIF from the sample, fewer sites are left to bind MIF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MIF concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq, Jul 2008]
Uniprot Description
MIF: a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. Also acts as a phenylpyruvate tautomerase.
Protein type: EC 5.3.3.12; EC 5.3.2.1; Apoptosis; Cell development/differentiation; Amino Acid Metabolism - tyrosine; Amino Acid Metabolism - phenylalanine; Isomerase; Cytokine
Chromosomal Location of Human Ortholog: 22q11.23
Cellular Component: nucleoplasm; extracellular space; cell surface; cytoplasm; extracellular region; vesicle
Molecular Function: phenylpyruvate tautomerase activity; protein binding; hematopoietin/interferon-class (D200-domain) cytokine receptor binding; cytokine activity; dopachrome isomerase activity; chemoattractant activity; receptor binding
Biological Process: DNA damage response, signal transduction by p53 class mediator; regulation of macrophage activation; carboxylic acid metabolic process; positive regulation of lipopolysaccharide-mediated signaling pathway; cell aging; negative regulation of cellular protein metabolic process; prostaglandin biosynthetic process; positive regulation of peptidyl-serine phosphorylation; cell proliferation; negative regulation of DNA damage response, signal transduction by p53 class mediator; positive regulation of MAP kinase activity; positive regulation of peptidyl-tyrosine phosphorylation; negative regulation of myeloid cell apoptosis; positive regulation of fibroblast proliferation; cell surface receptor linked signal transduction; negative regulation of mature B cell apoptosis; positive chemotaxis; positive regulation of B cell proliferation; innate immune response; inflammatory response; positive regulation of phosphorylation; negative regulation of apoptosis; positive regulation of cytokine secretion
Disease: Rheumatoid Arthritis, Systemic Juvenile
Research Articles on MIF
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Product Notes
The Guinea Pig MIF mif (Catalog #AAA720042) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA720042 ELISA Kit recognizes Guinea Pig MIF. It is sometimes possible for the material contained within the vial of "Macrophage MigRation Inhibitory Factor, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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