Microsomes Microsome
Microsomes, Ram Seminal Vessicles
Synonyms
Microsomes; Ram Seminal Vessicles; Ram Seminal Vesicle derived Microsomes; Microsomes microsome
Host
Microsomal fractions were derived from frozen ram seminal vesicles that were collected and placed at -80 degree C immediately after excision from post-slaughtered male ovine bucks
Specificity
Specific Activity: 169 pmol of metabolized Arachidonic Acid / mug protein / min at 37 degree C +/- 0.5 pmol
Concentration
5.0 mg/mL total protein using the BCA protein assay with BSA as a standard. (varies by lot)
Application Notes
This microsomal fractionation can be used as a source of active eicosanoid metabolizing enzymes. Many eicosanoids can be solubilized using the following protocol:
NOTE - All steps should be performed on ice, using ice cold solutions, or at 4 degree C where appropriate.
1) Ultracentrifuge suspended microsomes at 110,000 x g for 90 minutes and discard the resulting supernatant.
2) Resuspend microsome pellet in 2-4 x volume of 10 mM Tis-HCl; 0.5 mM EDTA; 1% Tween 20; pH to 8.0 using deoxygenated water with 3 x strokes of a Teflon pestle homogenizer.
3) Stir suspension for 45 minutes.
4) Ultracentrifuge the stirred suspension of microsomes at 100,000 x g for 90 minutes and collect the supernatant of solubilized eicosanoids. The solubilized eicosanoids are now ready for further processing or aliquot and storage at -80 degree C.
NOTE - All steps should be performed on ice, using ice cold solutions, or at 4 degree C where appropriate.
1) Ultracentrifuge suspended microsomes at 110,000 x g for 90 minutes and discard the resulting supernatant.
2) Resuspend microsome pellet in 2-4 x volume of 10 mM Tis-HCl; 0.5 mM EDTA; 1% Tween 20; pH to 8.0 using deoxygenated water with 3 x strokes of a Teflon pestle homogenizer.
3) Stir suspension for 45 minutes.
4) Ultracentrifuge the stirred suspension of microsomes at 100,000 x g for 90 minutes and collect the supernatant of solubilized eicosanoids. The solubilized eicosanoids are now ready for further processing or aliquot and storage at -80 degree C.
Storage Buffer
100 mM Sodium Phosphate, 250 mM Mannitol; 1 mM PMSF; 10 mM EDTA; pH 7.8
Preparation and Storage
At -80 degree C AVOID MULTIPLE FREEZE-THAW CYCLES.
Related Product Information for Microsomes microsome
Description: Eicosanoid metabolizing enzymes such as cycloxygenases, prostaglandins, and numerous other enzymes, are microsomal membrane associated and are found highly expressed in seminal vesicle tissues. This microsomal fraction has been isolated from ram seminal vesicles with care to minimize the loss of enzymatic activity.
Similar Products
Product Notes
The Microsomes (Catalog #AAA480530) is a Microsome produced from Microsomal fractions were derived from frozen ram seminal vesicles that were collected and placed at -80 degree C immediately after excision from post-slaughtered male ovine bucks and is intended for research purposes only. The product is available for immediate purchase. This microsomal fractionation can be used as a source of active eicosanoid metabolizing enzymes. Many eicosanoids can be solubilized using the following protocol: NOTE - All steps should be performed on ice, using ice cold solutions, or at 4 degree C where appropriate. 1) Ultracentrifuge suspended microsomes at 110,000 x g for 90 minutes and discard the resulting supernatant. 2) Resuspend microsome pellet in 2-4 x volume of 10 mM Tis-HCl; 0.5 mM EDTA; 1% Tween 20; pH to 8.0 using deoxygenated water with 3 x strokes of a Teflon pestle homogenizer. 3) Stir suspension for 45 minutes. 4) Ultracentrifuge the stirred suspension of microsomes at 100,000 x g for 90 minutes and collect the supernatant of solubilized eicosanoids. The solubilized eicosanoids are now ready for further processing or aliquot and storage at -80 degree C. Researchers should empirically determine the suitability of the Microsomes for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Microsomes, Microsome" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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