N-Acetyl Beta-D-Glucosaminidase (NAGase) Recombinant Protein | NAGase recombinant protein

Recombinant N-Acetyl Beta-D-Glucosaminidase (NAGase)

Cat. Num. AAA2010226

• Gene Names: Mgea5; Ncoat; O-GLCNACASE
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: N-Acetyl Beta-D-Glucosaminidase (NAGase); Recombinant N-Acetyl Beta-D-Glucosaminidase (NAGase); NAGase recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF- IKRAPVIWDN IHANDYDQKR LFLGPYKGRS TELIPRLKGV LTNPNCEFEA NYVAIHTLAT WYKSNMNGVR KDVVMTDSED STVSIQIKLE NEGSDEDIET DVLYSPQMAL KLALTEWLQE FGVPHQYSSR QVAHSGAKTS VVDGTPLVAA PSLNATTVVT TVYQEPIM
• Sequence Length: 916

• Applicable Applications for NAGase recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Ile271~Met438 (Accession # Q8VIJ5) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 18777747
• NCBI GeneID: 154968
• NCBI Accession #: NP_571979.1
• NCBI GenBank Nucleotide #: NM_131904.1
• UniProt Accession #: Q8VIJ5
• Molecular Weight: 20.3kDa
• NCBI Official Full Name: bifunctional protein NCOAT
• NCBI Official Synonym Full Names: meningioma expressed antigen 5 (hyaluronidase)
• NCBI Official Symbol: Mgea5
• NCBI Official Synonym Symbols: Ncoat; O-GLCNACASE
• NCBI Protein Information: bifunctional protein NCOAT; meningioma-expressed antigen 5; nuclear cytoplasmic O-GlcNAcase and acetyltransferase
• UniProt Protein Name: Protein O-GlcNAcase
• UniProt Gene Name: Mgea5
• UniProt Synonym Gene Names: OGA
• UniProt Entry Name: NCOAT_RAT

NCBI Description

plays a role in insulin secretion; may be involved in secretion of other glucose regulated hormones [RGD, Feb 2006]

Uniprot Description

OGA: Cleaves GlcNAc but not GalNAc from glycopeptides. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl- beta-GalNAc or p-nitrophenyl-alpha-GlcNAc. Possesses hyaluronidase activity. Acetylates 'Lys-8' of histone H4 and 'Lys-14' of histone H3. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.2.1.52; Acetyltransferase; Hydrolase; EC 3.2.1.169

Cellular Component: membrane; mitochondrion; cytosol; nucleus

Molecular Function: beta-N-acetylglucosaminidase activity; histone acetyltransferase activity

Biological Process: positive regulation of DNA metabolic process; positive regulation of protein complex disassembly; dATP metabolic process; positive regulation of proteolysis; protein deglycosylation; N-acetylglucosamine metabolic process; positive regulation of insulin secretion; positive regulation of growth hormone secretion; positive regulation of glucose import; negative regulation of protein amino acid glycosylation; positive regulation of mitochondrial depolarization; response to steroid hormone stimulus; positive regulation of cell killing; histone acetylation; protein targeting to membrane; positive regulation of calcium ion transport; aging