MFG-E8 recombinant protein

Recombinant Human MFG-E8 Protein

Cat. Num. AAA286116

• Gene Names: MFGE8; BA46; HMFG; MFGM; SED1; hP47; EDIL1; MFG-E8; SPAG10; OAcGD3S; HsT19888
• Purity: > 90 % as determined by SDS-PAGE.
• Synonyms: MFG-E8; Recombinant Human MFG-E8 Protein; BA46; EDIL1; HMFG; hP47; HsT19888; MFGMOAcGD3S; SED1; SPAG10; MFG-E8 recombinant protein

• Host: E Coli
• Purity/Purification: > 90 % as determined by SDS-PAGE.
• Form/Format: 100 mM Tris-HCI, 0.4 M Arg, pH 8.0, with 50% glycerol.
• Concentration: 2.0 mg/mL (varies by lot)
• Sequence Positions: 24-387
• Sequence: LDICSKNPCHNGGLCEEISQEVRGDVFPSYTCTCLKGYAGNHCETKCVEPLGMENGNIANSQIAASSVRVTFLGLQHWVPELARLNRAGMVNAWTPSSNDDNPWlQVNLLRRMWVTGVVTQGASRLASHEYLKAFKVAYSLNGHEFDFIHDVNKKHKEFVGNVVNKNAVHVNLFETPVEAQYVRLYPTSCHTACTLRFELLGCELNGCANPLGLKNNSIPDKQITASSSYKTVVGLHLFSVVNPSYARLDKQGNFNAVVVAGSYGNDQVVLQVDLGSSKEVTGIITQGARNFGSVQFVASYKVAYSNDSANVVTEYQDPRTGSSKIFPGNVVDNHSHKKNLFETPILARYVRILPVAVVHNRIALRLELLGC

• Species: Human
• Source: Human
• Protein Residue: N-Terminal 6*His-SUMO-tagged
• Usage: MFGEB Protein - Centrifuge the standard vial at 6000-10000rpm for 30s.
• Preparation and Storage: Store it under sterile conditions at -20°C to -80°C upon receiving.; Recommend to aliquot the protein into smaller quantities for optimal storage.; **Avoid repeated freeze-thaw cycles.

SDS-PAGE

Related Product Information for MFG-E8 recombinant protein

N/AMFGE8 is a protein which is encoded by the MFGE8 gene. MFGE8 is secreted protein found in vertebrates, including mammals as well as birds.Mfge8 contains a phosphatidylserine (PS) binding domain, as well as an Arginine-GlycineAspartic acid motif, which enables the binding to integrins. MFGE8 binds PS, which is exposed on the surface of apoptotic cells. Opsonization of the apoptotic cells and binding to integrins on the surface of phagocytic cells, mediates the engulfment of the dead cell. MFGE8, when engaged by phospholipids, bound to cells via its RGD motif. It bound particularly strongly to cells expressing alpha-V-beta-3 integrin. The NIH 3T3 cell transformants that expressed a high level of alpha-V-beta-3 integrin engulfed apoptotic cells when MFGE8 was added.

NCBI and Uniprot Product Information

• NCBI GI #: 167830475
• NCBI GeneID: 4240
• NCBI Accession #: NP_005919.2
• NCBI GenBank Nucleotide #: NM_005928.3
• UniProt Accession #: Q08431; Q53FU9; Q7Z3D2; Q9BTL9; B2R6M7
• Molecular Weight: Predicted MW: 56.8 kDa; Observed MW: 57 kDa
• NCBI Official Full Name: lactadherin isoform a preproprotein
• NCBI Official Synonym Full Names: milk fat globule-EGF factor 8 protein
• NCBI Official Symbol: MFGE8
• NCBI Official Synonym Symbols: BA46; HMFG; MFGM; SED1; hP47; EDIL1; MFG-E8; SPAG10; OAcGD3S; HsT19888
• NCBI Protein Information: lactadherin
• UniProt Protein Name: Lactadherin
• UniProt Gene Name: MFGE8
• UniProt Synonym Gene Names: MFG-E8
• UniProt Entry Name: MFGM_HUMAN

NCBI Description

This gene encodes a preproprotein that is proteolytically processed to form multiple protein products. The major encoded protein product, lactadherin, is a membrane glycoprotein that promotes phagocytosis of apoptotic cells. This protein has also been implicated in wound healing, autoimmune disease, and cancer. Lactadherin can be further processed to form a smaller cleavage product, medin, which comprises the major protein component of aortic medial amyloid (AMA). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]

Uniprot Description

MFGE8: Plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization. Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction. Binds specifically to rotavirus and inhibits its replication. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 15q25

Cellular Component: extracellular matrix; extracellular region; extracellular space; membrane

Biological Process: cellular protein metabolic process