Specificity
This assay has high sensitivity and excellent specificity for detection of Human MET. No significant cross-reactivity or interference between Human MET and analogues was observed.
Samples
Serum, Plasma, Other biological fluids
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for MET elisa kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate MET in samples. An antibody specific for MET has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMET present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MET is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MET bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GenBank Nucleotide #
Molecular Weight
85,745 Da
NCBI Official Full Name
hepatocyte growth factor receptor isoform b preproprotein
NCBI Official Synonym Full Names
MET proto-oncogene, receptor tyrosine kinase
NCBI Official Synonym Symbols
HGFR; AUTS9; RCCP2; c-Met; DFNB97
NCBI Protein Information
hepatocyte growth factor receptor
UniProt Protein Name
Hepatocyte growth factor receptor
UniProt Synonym Gene Names
HGF receptor; SF receptor
NCBI Description
This gene encodes a member of the receptor tyrosine kinase family of proteins and the product of the proto-oncogene MET. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that are linked via disulfide bonds to form the mature receptor. Further processing of the beta subunit results in the formation of the M10 peptide, which has been shown to reduce lung fibrosis. Binding of its ligand, hepatocyte growth factor, induces dimerization and activation of the receptor, which plays a role in cellular survival, embryogenesis, and cellular migration and invasion. Mutations in this gene are associated with papillary renal cell carcinoma, hepatocellular carcinoma, and various head and neck cancers. Amplification and overexpression of this gene are also associated with multiple human cancers. [provided by RefSeq, May 2016]
Uniprot Description
Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling plays a role in gastrulation, development and migration of muscles and neuronal precursors, angiogenesis and kidney formation. In adults, participates in wound healing as well as organ regeneration and tissue remodeling. Promotes also differentiation and proliferation of hematopoietic cells. May regulate cortical bone osteogenesis ().
Research Articles on MET
Similar Products
Product Notes
The Human MET met (Catalog #
AAA285228) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA285228 ELISA Kit recognizes Human MET.
It is sometimes possible for the material contained within the vial of
"Hepatocyte growth factor receptor, ELISA Kit" to become dispersed throughout the inside of
the vial, particularly around the seal of said vial, during shipment and storage. We always
suggest centrifuging these vials
to consolidate all of the liquid away from the lid and to the bottom of the vial prior to
opening. Please be advised that
certain products may require dry ice for shipping and that, if this is the case, an
additional dry ice fee may also be
required.
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All products in the AAA Biotech catalog are strictly for research-use only, and are
absolutely not suitable for use in any
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