Typical Testing Data/Standard Curve
Mouse Meprin A Alpha ELISA Kit | MEP1a elisa kit
Mouse Meprin A Alpha (MEP1a) ELISA Kit
Gene Names
MEP1A; PPHA
Reactivity
Mouse
Synonyms
Meprin A Alpha; Mouse Meprin A Alpha (MEP1a) ELISA Kit; PPHA; PABA Peptide Hydrolase; Endopeptidase-2; N-benzoyl-L-tyrosyl-P-amino-Benzoic Acid Hydrolase Subunit Alpha; MEP1a elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Meprin A Alpha (MEP1a).
No significant cross-reactivity or interference between Meprin A Alpha (MEP1a) and analogues was observed.
No significant cross-reactivity or interference between Meprin A Alpha (MEP1a) and analogues was observed.
Sequence Length
746
Assay Type
Competitive Inhibition
Samples
Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
Detection Range
49.4-4,000pg/mL
Sensitivity
< 19.1pg/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Meprin A Alpha (MEP1a) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Meprin A Alpha (MEP1a) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve
Typical Testing Data/Standard Curve
Related Product Information for MEP1a elisa kit
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Meprin A Alpha (MEP1a) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Meprin A Alpha (MEP1a) and unlabeled Meprin A Alpha (MEP1a) (Standards or samples) with the pre-coated antibody specific to Meprin A Alpha (MEP1a). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Meprin A Alpha (MEP1a) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Meprin A Alpha (MEP1a) in the sample.
Product Categories/Family for MEP1a elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
84,419 Da
NCBI Official Full Name
meprin A subunit alpha
NCBI Official Synonym Full Names
meprin A subunit alpha
NCBI Official Symbol
MEP1A
NCBI Official Synonym Symbols
PPHA
NCBI Protein Information
meprin A subunit alpha
UniProt Protein Name
Meprin A subunit alpha
Protein Family
UniProt Gene Name
MEP1A
UniProt Entry Name
MEP1A_HUMAN
Uniprot Description
MEP1A: Belongs to the peptidase M12A family.
Protein type: Membrane protein, integral; Protease; EC 3.4.24.18
Chromosomal Location of Human Ortholog: 6p12-p11
Cellular Component: extracellular space; integral to plasma membrane; meprin A complex
Molecular Function: metalloendopeptidase activity; protein binding; zinc ion binding
Biological Process: digestion; proteolysis
Research Articles on MEP1a
Similar Products
Product Notes
The Mouse MEP1a mep1a (Catalog #AAA2031978) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2031978 ELISA Kit recognizes Mouse MEP1a. It is sometimes possible for the material contained within the vial of "Meprin A Alpha, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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