Mediator Complex Subunit 8 (MED8) Recombinant Protein | MED8 recombinant protein

Recombinant Mediator Complex Subunit 8 (MED8)

Cat. Num. AAA2086879

• Gene Names: MED8; ARC32
• Applications: SDS-Page, Western Blot
• Purity: > 95%
• Synonyms: Mediator Complex Subunit 8 (MED8); Recombinant Mediator Complex Subunit 8 (MED8); ARC32; Mediator Of RNA Polymerase II Transcription Subunit 8; Activator-recruited cofactor 32 kDa component; MED8 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Freeze-dried powder
• Sequence Length: 179

• Applicable Applications for MED8 recombinant protein: Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)
• Fragment: Ser25~Arg268
• Tag: N-terminal His-tag
• Buffer Formulation: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Source Note: Prokaryotic expression
• Organism Species: Human
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Isoelectric Point: 8.5
• Subcellular Location: Nucleus.
• Usage: Reconstitute in PBS or others.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

Product Categories/Family for MED8 recombinant protein

Recombinant Protein

NCBI and Uniprot Product Information

• NCBI GI #: 48762704
• NCBI GeneID: 112950
• NCBI Accession #: NP_001001653.1
• NCBI GenBank Nucleotide #: NM_001001653.2
• UniProt Accession #: Q96G25; Q5JUY8; Q96FQ4; A9IZ91; A9IZ92
• Molecular Weight: 30.1kDa
• NCBI Official Full Name: mediator of RNA polymerase II transcription subunit 8 isoform 1
• NCBI Official Synonym Full Names: mediator complex subunit 8
• NCBI Official Symbol: MED8
• NCBI Official Synonym Symbols: ARC32
• NCBI Protein Information: mediator of RNA polymerase II transcription subunit 8
• UniProt Protein Name: Mediator of RNA polymerase II transcription subunit 8
• Protein Family: Mediator of RNA polymerase II transcription
• UniProt Gene Name: MED8
• UniProt Synonym Gene Names: ARC32

NCBI Description

This gene encodes a protein component of the mediator complex, which aids in transcriptional activation through interaction with RNA polymerase II and gene-specific transcription factors. The encoded protein may also function in ubiquitin ligation and protein degradation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2013]

Uniprot Description

MED8: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May play a role as a target recruitment subunit in E3 ubiquitin-protein ligase complexes and thus in ubiquitination and subsequent proteasomal degradation of target proteins. Belongs to the Mediator complex subunit 8 family. 3 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 1p34.2

Cellular Component: nucleoplasm; Srb-mediator complex

Molecular Function: protein binding

Biological Process: transcription initiation from RNA polymerase II promoter