Mouse anti-Human, Mouse TPX2 Monoclonal Antibody | anti-TPX2 antibody

TPX2 (P100, C20orf1, C20orf2, DIL2, HCA519, p100, Protein FLS353, Hepatocellular carcinoma-associated antigen 519, Targeting protein for Xklp2, Restricted expression proliferation associated protein 100, Differentially expressed in cancerous and noncancer

Cat. Num. AAA6121596

• Gene Names: TPX2; DIL2; p100; DIL-2; HCTP4; FLS353; HCA519; REPP86; C20orf1; C20orf2; GD:C20orf1
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography
• Synonyms: TPX2; Monoclonal Antibody; TPX2 (P100; C20orf1; C20orf2; DIL2; HCA519; p100; Protein FLS353; Hepatocellular carcinoma-associated antigen 519; Targeting protein for Xklp2; Restricted expression proliferation associated protein 100; Differentially expressed in cancerous and noncancer; anti-TPX2 antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 10i76
• Specificity: Recognizes human P100. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).

• Applicable Applications for anti-TPX2 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Four KLH conjugated synthetic peptides selected from human P100.
• Conjugate: HRP
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western analysis of extracts from Jurkat cells using P100 antibody.)

Product Categories/Family for anti-TPX2 antibody

Antibodies; Cancer Markers

References

1. Ota, T., et al., Nat. Genet. 36(1):40-45 (2004). 2.Heidebrecht, H.J., et al., Mol. Cancer Res. 1(4):271-279 (2003). 3.Garrett, S., et al., Curr. Biol. 12(23):2055-2059 (2002). 4.Gruss, O.J., et al., Nat. Cell Biol. 4(11):871-879 (2002). 5.Kufer, T.A., et al., J. Cell Biol. 158(4):617-623 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 20127519
• NCBI GeneID: 22974
• NCBI Accession #: NP_036244
• NCBI GenBank Nucleotide #: NM_012112
• UniProt Accession #: Q9ULW0
• Molecular Weight: 85,653 Da
• NCBI Official Full Name: targeting protein for Xklp2
• NCBI Official Synonym Full Names: TPX2, microtubule-associated
• NCBI Official Symbol: TPX2
• NCBI Official Synonym Symbols: DIL2; p100; DIL-2; HCTP4; FLS353; HCA519; REPP86; C20orf1; C20orf2; GD:C20orf1
• NCBI Protein Information: targeting protein for Xklp2; TPX2, microtubule-associated protein homolog; TPX2, microtubule-associated, homolog; differentially expressed in cancerous and non-cancerous lung cells 2; differentially expressed in lung cells; hepatocellular carcinoma-associ
• UniProt Protein Name: Targeting protein for Xklp2
• Protein Family: Targeting protein
• UniProt Gene Name: TPX2
• UniProt Synonym Gene Names: C20orf1; C20orf2; DIL2; HCA519; DIL-2; p100
• UniProt Entry Name: TPX2_HUMAN

Uniprot Description

TPX2: a microtubule-associated protein required for meiotic and mitotic spindle assembly. Targets a plus end-directed motor (XKLP2) to the minus ends of microtubules in the mitotic spindle. Required for microtubule nucleation around chromosomes in response to the local generation of RanGTP. Binds to and activates Aurora A kinase and targets it to the mitotic spindle.

Protein type: Microtubule-binding; Cell cycle regulation

Chromosomal Location of Human Ortholog: 20q11.2

Cellular Component: nucleoplasm; microtubule cytoskeleton; axon hillock; spindle pole; microtubule; cytoplasm; spindle; nucleus

Molecular Function: protein binding; GTP binding; protein kinase binding; ATP binding

Biological Process: mitosis; cell proliferation; apoptosis; cell division; activation of protein kinase activity