Mediator of RNA polymerase II transcription subunit 1 (MED1) Recombinant Protein | MED1 recombinant protein

Recombinant Human Mediator of RNA polymerase II transcription subunit 1 (MED1), partial

Cat. Num. AAA9424185

• Gene Names: MED1; PBP; CRSP1; RB18A; TRIP2; PPARBP; CRSP200; DRIP205; DRIP230; PPARGBP; TRAP220
• Reactivity: Human
• Purity: Greater than 90% as determined by SDS-PAGE.
• Synonyms: Mediator of RNA polymerase II transcription subunit 1 (MED1); Recombinant Human Mediator of RNA polymerase II transcription subunit 1 (MED1); partial; Activator-recruited cofactor 205 kDa component; MED1 recombinant protein

• Host: E Coli
• Reactivity: Human
• Purity/Purification: Greater than 90% as determined by SDS-PAGE.
• Form/Format: Tris-based buffer, 50% glycerol
• Sequence Positions: Partial, 878-1031aa

• Tag Info: N-terminal 6xHis-SUMO-tagged
• Preparation and Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.; Generally, the shelf life of liquid form is 6 months at -20 degree C/-80 degree C. The shelf life of lyophilized form is 12 months at -20 degree C/-80 degree C.

Related Product Information for MED1 recombinant protein

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (PubMed:10406464, PubMed:11867769, PubMed:12037571, PubMed:12218053, PubMed:12556447, PubMed:14636573, PubMed:15340084, PubMed:15471764, PubMed:15989967, PubMed:16574658, PubMed:9653119). Acts as a coactivator for GATA1-mediated transcriptional activation during erythroid differentiation of K562 erythroleukemia cells (PubMed:24245781).

NCBI and Uniprot Product Information

• NCBI GI #: 28559039
• NCBI GeneID: 5469
• NCBI Accession #: NP_004765.2
• NCBI GenBank Nucleotide #: NM_004774.3
• UniProt Accession #: Q15648; O43810; O75447; Q6P9H7; Q6PK58; Q9HD39; A2RRQ6
• Molecular Weight: 32.22 kDa
• NCBI Official Full Name: mediator of RNA polymerase II transcription subunit 1
• NCBI Official Synonym Full Names: mediator complex subunit 1
• NCBI Official Symbol: MED1
• NCBI Official Synonym Symbols: PBP; CRSP1; RB18A; TRIP2; PPARBP; CRSP200; DRIP205; DRIP230; PPARGBP; TRAP220
• NCBI Protein Information: mediator of RNA polymerase II transcription subunit 1
• UniProt Protein Name: Mediator of RNA polymerase II transcription subunit 1
• Protein Family: Mediator of RNA polymerase II transcription
• UniProt Gene Name: MED1
• UniProt Synonym Gene Names: ARC205; CRSP1; CRSP200; DRIP205; DRIP230; PBP; PPARBP; PPARGBP; RB18A; TRAP220; TRIP2; ARC205; PBP; PPAR-binding protein; Trap220; TR-interacting protein 2; TRIP-2

NCBI Description

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. It also regulates p53-dependent apoptosis and it is essential for adipogenesis. This protein is known to have the ability to self-oligomerize. [provided by RefSeq, Jul 2008]

Uniprot Description

MED1: a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. Also is a component of other multisubunit complexes e.g. thyroid hormone receptor- (TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. May regulates p53-dependent apoptosis. Is essential for adipogenesis. This protein is known to have the ability to self-oligomerize. Two splice-variant isoforms have been described.

Protein type: Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: membrane; nucleolus; nucleoplasm; nucleus; Srb-mediator complex

Molecular Function: chromatin binding; estrogen receptor binding; LBD domain binding; ligand-dependent nuclear receptor binding; ligand-dependent nuclear receptor transcription coactivator activity; nuclear hormone receptor binding; peroxisome proliferator activated receptor binding; protein binding; receptor activity; retinoic acid receptor binding; thyroid hormone receptor binding; thyroid hormone receptor coactivator activity; transcription coactivator activity; transcription cofactor activity; transcription factor binding; vitamin D receptor binding

Biological Process: androgen biosynthetic process; androgen receptor signaling pathway; angiogenesis; cell morphogenesis; erythrocyte development; fat cell differentiation; keratinocyte differentiation; lens development in camera-type eye; mRNA transcription from RNA polymerase II promoter; negative regulation of apoptosis; negative regulation of neuron differentiation; negative regulation of transcription from RNA polymerase II promoter; positive regulation of erythrocyte differentiation; positive regulation of keratinocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of lipid metabolic process; regulation of transcription from RNA polymerase I promoter; steroid hormone receptor signaling pathway; transcription initiation from RNA polymerase II promoter