Methyl CpG Binding Protein 2 Recombinant Protein | MECP2 recombinant protein

Recombinant Human Methyl CpG Binding Protein 2

Cat. Num. AAA144922

• Gene Names: MECP2; RS; RTS; RTT; PPMX; MRX16; MRX79; MRXSL; AUTSX3; MRXS13
• Purity: Greater than 80% as determined by SDS-PAGE.
• Synonyms: Methyl CpG Binding Protein 2; Recombinant Human Methyl CpG Binding Protein 2; MECP2 Human; Methyl CpG Binding Protein 2 Human Recombinant; Methyl CpG binding protein 2 (Rett syndrome); MeCp-2 protein; AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX; RTT; Mental retardation; X-linked 16; DKFZp686A24160; MECP2 recombinant protein

• Host: Mammalian system, 293 cells
• Purity/Purification: Greater than 80% as determined by SDS-PAGE.
• Form/Format: The MECP2 solution (0.45mg/ml) contains 50mM Tris, 135mM NaCl, 20% Glycerol, pH 7.5 and 200 ug/ml DYKDDDDK peptide.; Sterile Filtered colorless solution.
• Sequence: MDYKDDDDKMVAGMLGLREEKSEDQDLQGLKDKPLKFKKVKKDKKEEKEGKHEPVQPSAHHSAEPAEAGKAETSEGSGSAPAVPEASASPKQRRSIIRDRGPMYDDPTLPEGWTRKLKQRKSGRSAGKYDVYLINPQGKAFRSKVELIAYFEKVGDTSLDPNDFDFTVTGRGSPSRREQKPPKKPKSPKAPGTGRGRGRPKGSGTTRPKAATSEGVQVKRVLEKSPGKLLVKMPFQTSPGGKAEGGGATTSTQVMVIKRPGRKRKAEADPQAIPKKRGRKPGSVVAAAAAEAKKKAVKESSIRSVQETVLPIKKRKTRETVSIEVKEVVKPLLVSTLGEKSGKGLKTCKSPGRKSKESSPKGRSSSASSPPKKEHHHHHHHSESPKAPVPLLPPLPPPPPEPESSEDPTSPPEPQDLSSSVCKEEKMPRGGSLESDGCPKEPAKTQPAVATAATAAEKYKHRGEGERKDIVSSSMPRPNREEPVDSRTPVTERVS
• Sequence Length: 498

• Preparation and Storage: MECP2 although stable 4 degree C for 4 weeks, should be stored below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Related Product Information for MECP2 recombinant protein

Description: MECP2 Human Recombinant is expressed in 293 cells. The protein contains 486 amino acids (1-486a.a.) and fused to an N-terminal DYKDDDDK tag, having an Mw of 53.56kDa.

Introduction: MECP2 is the key modificator of eukaryotic genomes and has a crucial part in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 form a family of nuclear proteins linked by the existence in each of a methyl-CpG binding domain (MBD). Each one of these proteins, with the exception of MBD3, can bind specifically to methylated DNA. In addition, MECP2, MBD1 and MBD2 can inhibit transcription from methylated gene promoters. Unlike other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is expendable in stem cells, but is vital for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common reasons of mental retardation in females.

Product Categories/Family for MECP2 recombinant protein

Recombinant & Natural Proteins; Recombinant Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 160707950
• NCBI GeneID: 4204
• NCBI Accession #: NP_001104262.1
• NCBI GenBank Nucleotide #: NM_001110792.1
• UniProt Accession #: P51608; O15233; Q6QHH9; Q7Z384
• Molecular Weight: 53,323 Da
• NCBI Official Full Name: methyl-CpG-binding protein 2 isoform 2
• NCBI Official Synonym Full Names: methyl CpG binding protein 2
• NCBI Official Symbol: MECP2
• NCBI Official Synonym Symbols: RS; RTS; RTT; PPMX; MRX16; MRX79; MRXSL; AUTSX3; MRXS13
• NCBI Protein Information: methyl-CpG-binding protein 2; meCp-2 protein
• UniProt Protein Name: Methyl-CpG-binding protein 2
• Protein Family: Methyl-CpG-binding protein
• UniProt Gene Name: MECP2
• UniProt Synonym Gene Names: MeCp-2 protein; MeCp2
• UniProt Entry Name: MECP2_HUMAN

NCBI Description

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. [provided by RefSeq, Jul 2009]

Uniprot Description

MECP2: Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Interacts with FNBP3. Interacts with CDKL5. Present in all adult somatic tissues tested. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: nucleoplasm; heterochromatin; extracellular space; cytosol; nucleus

Molecular Function: mRNA binding; protein domain specific binding; protein binding; siRNA binding; methyl-CpG binding; DNA binding; double-stranded methylated DNA binding; protein N-terminus binding; chromatin binding; transcription factor activity; transcription factor binding; transcription corepressor activity

Biological Process: catecholamine secretion; startle response; genetic imprinting; behavioral fear response; adult locomotory behavior; positive regulation of transcription, DNA-dependent; chromatin silencing; neurological control of breathing; pathogenesis; negative regulation of transcription from RNA polymerase II promoter; proprioception; sensory perception of pain; negative regulation of histone methylation; neuron maturation; post-embryonic development; negative regulation of histone acetylation; phosphatidylcholine metabolic process; protein localization; synaptogenesis; positive regulation of cell proliferation; dendrite development; cerebellum development; visual learning; histone acetylation; negative regulation of neuron apoptosis; mitochondrial electron transport, ubiquinol to cytochrome c; transcription, DNA-dependent; ventricular system development; glutamine metabolic process; respiratory gaseous exchange; social behavior; cardiolipin metabolic process; histone methylation; glucocorticoid metabolic process; positive regulation of synaptogenesis; regulation of systemic arterial blood pressure by neurological process; long-term memory; inositol metabolic process; response to hypoxia; regulation of excitatory postsynaptic membrane potential; negative regulation of transcription, DNA-dependent

Disease: Rett Syndrome; Mental Retardation, X-linked, Syndromic 13; Autism, Susceptibility To, X-linked 3; Lubs X-linked Mental Retardation Syndrome; Angelman Syndrome; Encephalopathy, Neonatal Severe, Due To Mecp2 Mutations