PRDM10 recombinant protein

Recombinant PRDM10 protein

Cat. Num. AAA388116

• Gene Names: MECOM; EVI1; MDS1; PRDM3; MDS1-EVI1; AML1-EVI-1
• Purity: The recombinant protein is >70% pure by SDS-PAGE.
• Synonyms: PRDM10; Recombinant PRDM10 protein; PFM7; PRDM10 recombinant protein

• Host: Baculovirus
• Purity/Purification: The recombinant protein is >70% pure by SDS-PAGE.
• Form/Format: Full length recombinant PRDM10 protein expressed in Sf9 cells and supplied in 25mM HEPES, pH7.5, 300mM NaCl, 0.2mg/ml 3X DYKDDDDK peptide and 5% glycerol.

• Protein Species: Human
• Tag: DYKDDDDK-Tag
• Notes: This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant PRDM10 protein gel. PRDM10 protein was run on an SDS-PAGE gel and stained with Coomassie Blue.)

Related Product Information for PRDM10 recombinant protein

Short Description: Recombinant PRDM10 (accession number NP_064613.2) was expressed in Sf9 cells and contains an N-terminal DYKDDDDK tag with an observed molecular weight of 134 kDa. PRDM10 (PR Domain Containing 10) is a member of the PRDI-BF1 and RIZ homology domain containing (PRDM) family of transcriptional regulators. PRDM10 is a transcription factor that contains C2H2-type zinc-fingers and that possesses histone methyltransferase activity. PRDM10 also contains a positive regulatory domain which has been found in several other zinc-finger transcription factors including those involved in B cell differentiation and tumor suppression. Studies of the mouse counterpart suggest that this protein may be involved in the development of the central nerve system (CNS), as well as in the pathogenesis of neuronal storage disease. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. Recombinant PRDM10 can be used to study enzyme kinetics and perform inhibitor screening assays.

Background: PRDM10 (PR Domain Containing 10) is a member of the PRDI-BF1 and RIZ homology domain containing (PRDM) family of transcriptional regulators. PRDM10 is a transcription factor that contains C2H2-type zinc-fingers and that possesses histone methyltransferase activity. PRDM10 also contains a positive regulatory domain which has been found in several other zinc-finger transcription factors including those involved in B cell differentiation and tumor suppression. Studies of the mouse counterpart suggest that this protein may be involved in the development of the central nerve system (CNS), as well as in the pathogenesis of neuronal storage disease. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed.

Product Categories/Family for PRDM10 recombinant protein

Recombinant Proteins; Histone Methyltransferase (HMT); Transcription

NCBI and Uniprot Product Information

• NCBI GI #: 255683386
• NCBI GeneID: 2122
• NCBI Accession #: NP_004982.2
• NCBI GenBank Nucleotide #: NM_004991.3
• UniProt Accession #: Q13465; Q13466; Q6FH90; Q03112
• Molecular Weight: 18,696 Da
• NCBI Official Full Name: MDS1 and EVI1 complex locus protein EVI1 isoform c
• NCBI Official Synonym Full Names: MDS1 and EVI1 complex locus
• NCBI Official Symbol: MECOM
• NCBI Official Synonym Symbols: EVI1; MDS1; PRDM3; MDS1-EVI1; AML1-EVI-1
• NCBI Protein Information: MDS1 and EVI1 complex locus protein EVI1; oncogene EVI1; zinc finger protein Evi1; AML1-EVI-1 fusion protein; MDS1 and EVI1 complex locus protein MDS1; myelodysplasia syndrome-associated protein 1; ecotropic virus integration site 1 protein homolog
• UniProt Protein Name: MDS1 and EVI1 complex locus protein MDS1
• Protein Family: PR domain zinc finger protein
• UniProt Gene Name: MECOM
• UniProt Synonym Gene Names: MDS1
• UniProt Entry Name: MDS1_HUMAN

NCBI Description

The protein encoded by this gene is a transcriptional regulator and oncoprotein that may be involved in hematopoiesis, apoptosis, development, and cell differentiation and proliferation. The encoded protein can interact with CTBP1, SMAD3, CREBBP, KAT2B, MAPK8, and MAPK9. This gene can undergo translocation with the AML1 gene, resulting in overexpression of this gene and the onset of leukemia. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Mar 2011]

Uniprot Description

MDS1: A chromosomal aberration involving MDS1 is found in a form of acute myeloid leukemia (AML). Translocation t(3;21) with AML1. 6 isoforms of the human protein are produced by alternative promoter.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 3q26.2

Cellular Component: nucleoplasm; Golgi apparatus; intracellular membrane-bound organelle; cytoplasm; histone deacetylase complex; nuclear speck; nucleus

Molecular Function: protein binding; protein homodimerization activity; DNA binding; metal ion binding; transcription factor activity

Biological Process: embryonic forelimb morphogenesis; pericardium development; negative regulation of JNK cascade; transcription, DNA-dependent; in utero embryonic development; regulation of cell cycle; apoptosis; positive regulation of transcription, DNA-dependent; neutrophil homeostasis; embryonic hindlimb morphogenesis; regulation of cell proliferation; post-embryonic development; regulation of transcription, DNA-dependent; response to bacterium; forebrain development; negative regulation of programmed cell death; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; cell differentiation; inflammatory response; negative regulation of transforming growth factor beta receptor signaling pathway