E3 ubiquitin-protein ligase Mdm2 (MDM2) Recombinant Protein | MDM2 recombinant protein

Recombinant Dog E3 ubiquitin-protein ligase Mdm2 (MDM2)

Cat. Num. AAA956225

• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: E3 ubiquitin-protein ligase Mdm2 (MDM2); Recombinant Dog E3 ubiquitin-protein ligase Mdm2 (MDM2); MDM2 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-487, Full length protein
• Sequence: MCNTNMSVSTGGAVSTSQIPASEQETLVRPKPLLLKLLKSVGAQKDTYTMKEVIFYLGQYIMTKRLYDEKQQHIVYCSNDLLGDLFGVPSFSVKEHRKIYTMIYRNLVVVNQHEPSDSGTSVSENSCHREGGSDQKDPVQELQEEKPSSSDLISRPSTSSRRRTISETEEHADDLPGERQRKRHKSDSISLSFDESLALCVIREICCERSSSSESTGTPSNPDLDAGVSEHSGDWLDQDSVSDQFSVEFEVESLDSEDYSLSEEGQELSDEDDEVYRVTVYQAGESDTDSFEEDPEISLADYWKCTSCNEMNPPLPPHCNRCWALRENWLPEDKGKIPEKATPENSTQVEEGFDVPDCKKAAASDSRESCAEEIDDKITQASHSQESEDYSQPSTSNSIIYSSQEDVKEFEREETQDKEEIVESSFPLNAIEPCVICQGRPKNGCIVHGKTGHLMACFTCAKKLKKRNKPCPVCRQPIQMIVLTYFP
• Sequence Length: 487

• Species: Canis familiaris (Dog) (Canis lupus familiaris)
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

Related Product Information for MDM2 recombinant protein

This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues.

NCBI and Uniprot Product Information

• NCBI GI #: 50978798
• NCBI GeneID: 403693
• NCBI Accession #: NP_001003103.1
• NCBI GenBank Nucleotide #: NM_001003103.2
• UniProt Accession #: P56950; Q95KN5
• Molecular Weight: 48,034 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase Mdm2
• NCBI Official Synonym Full Names: MDM2 proto-oncogene
• NCBI Official Symbol: MDM2
• NCBI Protein Information: E3 ubiquitin-protein ligase Mdm2
• UniProt Protein Name: E3 ubiquitin-protein ligase Mdm2
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: MDM2
• UniProt Synonym Gene Names: Cdm2

Uniprot Description

E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome. Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Also acts as a ubiquitin ligase E3 toward itself and ARRB1. Permits the nuclear export of p53/TP53. Promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma RB1 protein. Inhibits DAXX-mediated apoptosis by inducing its ubiquitination and degradation. Component of the TRIM28/KAP1-MDM2-p53/TP53 complex involved in stabilizing p53/TP53. Also component of the TRIM28/KAP1-ERBB4-MDM2 complex which links growth factor and DNA damage response pathways. Mediates ubiquitination and subsequent proteasome degradation of DYRK2 in nucleus. Ubiquitinates IGF1R and SNAI1 and promotes them to proteasomal degradation. Ubiquitinates DCX, leading to DCX degradation and reduction of the dendritic spine density of olfactory bulb granule cells. Ubiquitinates DLG4, leading to proteasomal degradation of DLG4 which is required for AMPA receptor endocytosis.