Rabbit anti-Human MDM2 Polyclonal Antibody | anti-MDM2 antibody

MDM2, phosphorylated (Thr218) (Ubiquitin-protein Ligase E3 Mdm2, p53-binding Protein Mdm2, Oncoprotein Mdm2, Double Minute 2 Protein, Hdm2)

Cat. Num. AAA630163

• Gene Names: MDM2; HDMX; hdm2; MGC5370; MGC71221
• Reactivity: Human
• Applications: ELISA, Gel Super Shift Assay
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: MDM2; Polyclonal Antibody; phosphorylated (Thr218) (Ubiquitin-protein Ligase E3 Mdm2; p53-binding Protein Mdm2; Oncoprotein Mdm2; Double Minute 2 Protein; Hdm2); Anti -MDM2; anti-MDM2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MDM2 when phosphorylated at Thr218.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-MDM2 antibody: ELISA (EL/EIA), Gel Shift Assay (GS/EMSA)
• Application Notes: Suitable for use in ELISA and Dot Blot.; Dilution: ELISA: 1:1,000; Dot Blot: 1:500
• Immunogen: Synthetic phosphopeptide corresponding to amino acid residues surrounding Thr218 of human MDM2 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Dot Blot (DB)

(Dot blot analysis of anti-hMDM2-T218 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

Related Product Information for anti-MDM2 antibody

MDM2 is a target of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of MDM2 can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle,apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5.

Product Categories/Family for anti-MDM2 antibody

Antibodies; Abs to Ubiquitin Ligases

NCBI and Uniprot Product Information

• NCBI GI #: 155183770
• NCBI GeneID: 4193
• UniProt Accession #: Q00987; Q13226; Q13297; Q13298; Q13299; Q13300; Q13301; Q53XW0; Q71TW9; Q8WYJ1; A6NL51; A8K2S6; Q546E6; Q86WA2; Q86WA3; Q86WA4; Q86WA5; Q8NDV9; Q8NDW0; Q8NDW1; Q8NDW2
• Molecular Weight: 55,233 Da
• NCBI Official Full Name: Mdm2
• NCBI Official Synonym Full Names: Mdm2 p53 binding protein homolog (mouse)
• NCBI Official Symbol: MDM2
• NCBI Official Synonym Symbols: HDMX; hdm2; MGC5370; MGC71221
• NCBI Protein Information: E3 ubiquitin-protein ligase Mdm2; oncoprotein Mdm2; MDM2 variant FB28; MDM2 variant FB30; OTTHUMP00000183488; OTTHUMP00000183489; OTTHUMP00000183495; OTTHUMP00000183496; OTTHUMP00000240280; OTTHUMP00000240285; OTTHUMP00000240290; ubiquitin-protein ligase E3 Mdm2; double minute 2, human homolog of; p53-binding protein; Mdm2, transformed 3T3 cell double minute 2, p53 binding protein
• UniProt Protein Name: E3 ubiquitin-protein ligase Mdm2
• Protein Family: Mdm2-binding protein
• UniProt Gene Name: MDM2
• UniProt Entry Name: MDM2_HUMAN

NCBI Description

This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues. [provided by RefSeq]

Uniprot Description

MDM2: a ubiquitin ligase for p53, plays a central role in regulation of the stability of p53 via Akt-mediated MDM2 phosphorylation. Phosphorylation of MDM2 increases its interaction with p300, providing a platform to allow the assembly of the protein complex necessary for MDM2-mediated ubiquitination and degradation of p53. Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53. Facilitates the nuclear export of p53 and targets it for proteasome-mediated proteolysis. Eight alternatively spliced isoforms have been reported.

Protein type: EC 6.3.2.19; Ubiquitin ligase; Ligase; Ubiquitin conjugating system; Oncoprotein; EC 6.3.2.-; Inhibitor; Nucleolus

Chromosomal Location of Human Ortholog: 12q14.3-q15

Cellular Component: nucleoplasm; nuclear body; protein complex; cytoplasm; plasma membrane; nucleolus; synapse; cytosol; nucleus

Molecular Function: identical protein binding; protein binding; peroxisome proliferator activated receptor binding; enzyme binding; zinc ion binding; p53 binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: viral reproduction; nerve growth factor receptor signaling pathway; protein ubiquitination during ubiquitin-dependent protein catabolic process; response to morphine; protein ubiquitination; negative regulation of transcription from RNA polymerase II promoter; negative regulation of caspase activity; positive regulation of mitotic cell cycle; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; response to carbohydrate stimulus; response to antibiotic; positive regulation of protein export from nucleus; negative regulation of DNA damage response, signal transduction by p53 class mediator; synaptic transmission; establishment of protein localization; response to magnesium ion; peptidyl-lysine modification; positive regulation of cell proliferation; protein complex assembly; response to iron ion; epidermal growth factor receptor signaling pathway; response to drug; traversing start control point of mitotic cell cycle; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; protein destabilization; response to cocaine; response to ether; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; innate immune response; regulation of protein catabolic process; negative regulation of transcription, DNA-dependent

Disease: Accelerated Tumor Formation, Susceptibility To