Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Monocarboxylate transporter 3 (SLC16A8) Standard Curve.)
Human Monocarboxylate Transporter 3 (SLC16A8) ELISA Kit | MCT3 elisa kit
Human Monocarboxylate Transporter 3 (SLC16A8) ELISA Kit
Gene Names
SLC16A8; MCT3; REMP
Reactivity
Human
Synonyms
Monocarboxylate Transporter 3 (SLC16A8); Human Monocarboxylate Transporter 3 (SLC16A8) ELISA Kit; SLC16A8; RP5-1039K5.14; REMP; monocarboxylate transporter 3; solute carrier 16 (monocarboxylic acid transporters); member 8; solute carrier family 16; MCT3 elisa kit
Reactivity
Human
Specificity
Human Monocarboxylate transporter 3 (SLC16A8) ELISA Kit has high sensitivity and excellent specificity for detection of Human SLC16A8. No significant cross-reactivity or interference between Human SLC16A8 and analogues was observed.
Sequence Length
504
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Monocarboxylate transporter 3 (SLC16A8) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Monocarboxylate transporter 3 (SLC16A8) Standard Curve.)
Related Product Information for MCT3 elisa kit
Description: This Human Monocarboxylate transporter 3 (SLC16A8) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC16A8 in samples. An antibody specific for SLC16A8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SLC16A8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC16A8 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC16A8 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: SLC16A8 is a member of a family of proton-coupled monocarboxylate transporters that mediate lactate transport across cell membranes. The deduced 504-amino acid protein has a calculated molecular mass of 52.3 kD. MCT3 has 12 putative transmembrane domains and a long cytoplasmic loop between transmembrane domains 6 and 7. MCT3 shares 85% and 62% identity with rat and chicken Mct3, respectively, and highest similarity is in the transmembrane region. PCR analysis detected MCT3 in RPE RNA, but not in neural retina RNA, suggesting that in human, as in chicken and rat, the expression of MCT3 is restricted to RPE.
Background: SLC16A8 is a member of a family of proton-coupled monocarboxylate transporters that mediate lactate transport across cell membranes. The deduced 504-amino acid protein has a calculated molecular mass of 52.3 kD. MCT3 has 12 putative transmembrane domains and a long cytoplasmic loop between transmembrane domains 6 and 7. MCT3 shares 85% and 62% identity with rat and chicken Mct3, respectively, and highest similarity is in the transmembrane region. PCR analysis detected MCT3 in RPE RNA, but not in neural retina RNA, suggesting that in human, as in chicken and rat, the expression of MCT3 is restricted to RPE.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
monocarboxylate transporter 3
NCBI Official Synonym Full Names
solute carrier family 16 member 8
NCBI Official Symbol
SLC16A8
NCBI Official Synonym Symbols
MCT3; REMP
NCBI Protein Information
monocarboxylate transporter 3
UniProt Protein Name
Monocarboxylate transporter 3
UniProt Gene Name
SLC16A8
UniProt Synonym Gene Names
MCT3; MCT 3
UniProt Entry Name
MOT3_HUMAN
NCBI Description
SLC16A8 is a member of a family of proton-coupled monocarboxylate transporters that mediate lactate transport across cell membranes (Yoon et al., 1999 [PubMed 10493836]).[supplied by OMIM, Apr 2010]
Uniprot Description
Function: Proton-linked monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate
By similarity.
Subcellular location: Cell membrane; Multi-pass membrane protein.
Tissue specificity: Retinal pigment epithelium.
Sequence similarities: Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family. [View classification]
Research Articles on MCT3
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Product Notes
The Human MCT3 slc16a8 (Catalog #AAA9714289) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714289 ELISA Kit recognizes Human MCT3. It is sometimes possible for the material contained within the vial of "Monocarboxylate Transporter 3 (SLC16A8), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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