Fc Fragment Of IgG Low Affinity IIIb Receptor (FcgR3B) Recombinant Protein | FcgR3B recombinant protein

Recombinant Fc Fragment Of IgG Low Affinity IIIb Receptor (FcgR3B)

Cat. Num. AAA2018785

• Gene Names: FCGR3B; CD16; FCG3; CD16b; FCGR3; FCR-10; FCRIII; FCRIIIb
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Fc Fragment Of IgG Low Affinity IIIb Receptor (FcgR3B); Recombinant Fc Fragment Of IgG Low Affinity IIIb Receptor (FcgR3B); FcgR3B recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- PKAVVFL EPQWYSVLEK DSVTLKCQGA YSPEDNSTQW FHNESLISSQ ASSYFIDAAT VNDSGEYRCQ TNLSTLSDPV QLEVHIGWLL LQAPRWVFKE EDPIHLRCHS WKNTALHKVT YLQNGKDRKY FHHNSDFHIPKATLKDSGSY FCRGLVGSKN VSSETVNITI TQGLAVSTIS SFS
• Sequence Length: 233

• Applicable Applications for FcgR3B recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Pro24~Ser203 (Accession # O75015) with two N-terminal Tags, His-tag and T7-tag
• Subcellular Location: Cell membrane; Lipid-anchor, GPI-anchor. Secreted
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 6.9
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 132814489
• NCBI GeneID: 2215
• NCBI Accession #: NP_000561.3
• NCBI GenBank Nucleotide #: NM_000570.4
• UniProt Accession #: O75015
• Molecular Weight: 24.2kDa
• NCBI Official Full Name: low affinity immunoglobulin gamma Fc region receptor III-B isoform 2
• NCBI Official Synonym Full Names: Fc fragment of IgG, low affinity IIIb, receptor (CD16b)
• NCBI Official Symbol: FCGR3B
• NCBI Official Synonym Symbols: CD16; FCG3; CD16b; FCGR3; FCR-10; FCRIII; FCRIIIb
• NCBI Protein Information: low affinity immunoglobulin gamma Fc region receptor III-B; fc-gamma RIII; fc-gamma RIIIb; fc-gamma RIII-beta; igG Fc receptor III-1; Fc-gamma receptor IIIb (CD 16); Fc fragment of IgG, low affinity IIIb, receptor for (CD16)
• UniProt Protein Name: Low affinity immunoglobulin gamma Fc region receptor III-B
• Protein Family: Low affinity immunoglobulin gamma Fc region receptor
• UniProt Gene Name: FCGR3B
• UniProt Synonym Gene Names: CD16B; FCG3; FCGR3; IGFR3; Fc-gamma RIII; Fc-gamma RIIIb; FcRIII; FcRIIIb
• UniProt Entry Name: FCG3B_HUMAN

NCBI Description

The protein encoded by this gene is a low affinity receptor for the Fc region of gamma immunoglobulins (IgG). The encoded protein acts as a monomer and can bind either monomeric or aggregated IgG. This gene may function to capture immune complexes in the peripheral circulation. Several transcript variants encoding different isoforms have been found for this gene. A highly-similar gene encoding a related protein is also found on chromosome 1. [provided by RefSeq, Aug 2012]

Uniprot Description

Function: Receptor for the Fc region of immunoglobulins gamma. Low affinity receptor. Binds complexed or aggregated IgG and also monomeric IgG. Contrary to III-A, is not capable to mediate antibody-dependent cytotoxicity and phagocytosis. May serve as a trap for immune complexes in the peripheral circulation which does not activate neutrophils.

Subunit structure: Monomer. Interacts with INPP5D/SHIP1

By similarity.

Subcellular location: Cell membrane; Lipid-anchor › GPI-anchor. Secreted. Note: Secreted after cleavage.

Tissue specificity: Expressed specifically by polymorphonuclear leukocytes (neutrophils). Also expressed by stimulated eosinophils.

Post-translational modification: Glycosylated. Glycosylation plays an inhibitory role in the interaction with IgG3.The soluble form is produced by a proteolytic cleavage.

Polymorphism: There are three allelic forms of FCGR3B: FCGR3B*01 (NA-1), FCGR3B*02 (HNA-1b, NA-2) (shown here) and SH. FCGR3B*01 and FCGR3B*02 are detectable with antibodies against the biallelic neutrophil-specific antigen system NA. The more active FCGR3B*01 allele has been associated with severe renal disease in certain systemic vasculitides.

Miscellaneous: Encoded by one of two nearly indentical genes: FCGR3A and FCGR3B (Shown here) which are expressed in a tissue-specific manner. The 'Phe-203' in FCGR3A determines the transmembrane domains whereas the Ser-203 in FCGR3B determines the GPI-anchoring.

Sequence similarities: Contains 2 Ig-like C2-type (immunoglobulin-like) domains.