Monkey Mast cell tryptase ELISA Kit | MCT elisa kit
Monkey Mast cell tryptase ELISA Kit
Reactivity
Monkey
Synonyms
Mast cell tryptase; Monkey Mast cell tryptase ELISA Kit; MCT elisa kit
Reactivity
Monkey
Specificity
This assay has high sensitivity and excellent specificity for detection of MCT. No significant cross-reactivity or interference between MCT and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MCT and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for MCT elisa kit
Intended Uses: This MCT ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey MCT. This ELISA kit for research use only!
Principle of the Assay: MCT ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-MCT antibody and an MCT-HRP conjugate. The assay sample and buffer are incubated together with MCT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MCT concentration since MCT from samples and MCT-HRP conjugate compete for the anti-MCT antibody binding site. Since the number of sites is limited, as more sites are occupied by MCT from the sample, fewer sites are left to bind MCT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MCT concentration in each sample is interpolated from this standard curve.
Principle of the Assay: MCT ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-MCT antibody and an MCT-HRP conjugate. The assay sample and buffer are incubated together with MCT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MCT concentration since MCT from samples and MCT-HRP conjugate compete for the anti-MCT antibody binding site. Since the number of sites is limited, as more sites are occupied by MCT from the sample, fewer sites are left to bind MCT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MCT concentration in each sample is interpolated from this standard curve.
Product Categories/Family for MCT elisa kit
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
54,994 Da
NCBI Official Full Name
MCT
UniProt Protein Name
Monocarboxylate transporter 6
Protein Family
UniProt Gene Name
SLC16A5
UniProt Synonym Gene Names
MCT5; MCT6; MCT 6; MCT 5
UniProt Entry Name
MOT6_HUMAN
Uniprot Description
SLC16A5: Proton-linked monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate. Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.
Protein type: Membrane protein, integral; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 17q25.1
Cellular Component: membrane; integral to plasma membrane
Molecular Function: monocarboxylic acid transmembrane transporter activity; symporter activity; lactate transmembrane transporter activity; secondary active monocarboxylate transmembrane transporter activity
Biological Process: monocarboxylic acid transport
