Monkey Melanin-concentrating hormone receptor 1 ELISA Kit | MCHR1 elisa kit

Monkey Melanin-concentrating hormone receptor 1 (MCHR1) ELISA Kit

Cat. Num. AAA286013

• Gene Names: MCHR1; GPR24; MCH1R
• Reactivity: Monkey
• Synonyms: Melanin-concentrating hormone receptor 1; Monkey Melanin-concentrating hormone receptor 1 (MCHR1) ELISA Kit; GPR24; MCH1R; MGC32129; SLC1; G protein-coupled receptor 24|G-protein coupled receptor 24; MCHR1 elisa kit

• Reactivity: Monkey
• Specificity: This assay has high sensitivity and excellent specificity for detection of Monkey MCHR1. No significant cross-reactivity or interference between Monkey MCHR1 and analogues was observed.
• Sequence Length: 422

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for MCHR1 elisa kit

Melanin-concentrating hormone receptor 1 is one of the melanin-concentrating hormone receptors.The protein encoded by this gene, a member of the G protein-coupled receptor family 1, is an integral plasma membrane protein which binds melanin-concentrating hormone. The encoded protein can inhibit cAMP accumulation and stimulate intracellular calcium flux, and is probably involved in the neuronal regulation of food consumption. Although structurally similar to somatostatin receptors, this protein does not seem to bind somatostatin.MCH1 is thought to have a number of functions including in the regulation of appetite, and in stress, anxiety and depression.Highest level in brain, particularly in the frontal cortex and hypothalamus, lower levels in the liver and heart.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate MCHR1 in samples. An antibody specific for MCHR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMCHR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MCHR1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MCHR1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 74136325
• NCBI GeneID: 574232
• NCBI Accession #: NP_001028057.1
• NCBI GenBank Nucleotide #: NM_001032885.1
• UniProt Accession #: Q8MJ89
• Molecular Weight: 42,777 Da
• NCBI Official Full Name: melanin-concentrating hormone receptor 1
• NCBI Official Symbol: MCHR1
• NCBI Official Synonym Symbols: GPR24; MCH1R
• NCBI Protein Information: melanin-concentrating hormone receptor 1
• UniProt Protein Name: Melanin-concentrating hormone receptor 1
• Protein Family: Melanin-concentrating hormone receptor
• UniProt Gene Name: MCHR1
• UniProt Synonym Gene Names: GPR24; MCH receptor 1; MCH-R1; MCHR-1; MCH1R; MCHR

Uniprot Description

Receptor for melanin-concentrating hormone, coupled to both G proteins that inhibit adenylyl cyclase and G proteins that activate phosphoinositide hydrolysis.